FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20639997
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG916590.44408436687272773TruSeq Adapter, Index 7 (97% over 34bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG633680.307015548500322No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG603110.2922044998359254No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC565640.2740504274298102No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT558770.2707219385739252No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA419560.20327522334426698No Hit
TGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG361080.17494188589271598No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG357460.17318800966879985No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC321270.1556540923915832No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA318900.15450583641073204No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT316520.15335273546793635No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG315600.15290699896904056No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC295590.1432122301180567No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA294390.14263083468471435No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG288730.13988858622411623No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT275310.13338664729457084No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG271590.13158432145120952No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT269530.13058625929063847No Hit
GGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCA266360.1290504063542257No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC264960.12837211168199297No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA257200.12461242121304572No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC255910.12398742112220268No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT245760.11906978474851522No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC240060.11630815644013903No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC237800.11521319504067756No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC226270.10962695391864641No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA222940.10801358159112134No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG214560.10395350348161388No Hit
GCACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTT208580.10105621623879113No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT206570.10008237888794266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG18900.043.7385252
TCGTATG130050.040.67244744-45
TATGCCG125100.040.09718346-47
TATCTCG125150.037.6727140-41
CGTACGG22000.037.359423
CGTATCT126500.037.3093138-39
AATTCGT142600.036.99514434-35
TCGTATC122050.036.41246436-37
GTATGCC145750.036.40423246-47
TCTCGTA133250.035.54110742-43
ATGCCGT150100.034.969248-49
GCCGTCT149400.034.86160750-51
CGAATTC154300.034.5111632-33
ACGAATT147900.033.9320130-31
CGTATGC150150.033.34538344-45
CACGAAT159950.033.15757830-31
ATCTCGT136000.032.88582240-41
GAATTCG156600.032.15392332-33
GTCACGA166050.032.0777228-29
TTCGTAT150100.031.74419636-37
Produced by FastQC (version 0.11.5)