FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20639997
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC712920.3454070269486958Illumina Single End PCR Primer 1 (96% over 31bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT506020.24516476431658396No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG498350.24144867850513738No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC380700.18444770122786355No Hit
GCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTT292750.1418362609258131No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGA254100.12311048301024462No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG245960.11916668398740562No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA238150.11538276870873576No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG237740.11518412526901045No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT237060.11485466785678311No Hit
TGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGAT230640.1117442022884015No Hit
GGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT230240.1115504038106207No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT229810.11134207044700636No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG222690.10789245754250837No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC208700.10111435578212535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG158400.047.0027242
CGTATCA94300.040.27323560-61
CGCCGTA86450.038.82444856-57
GTGGCGC213400.035.3364031
GTAGATC140950.034.08511742-43
TCGCCGT102900.034.07211756-57
GGCGCGT219500.033.942273
GGCGATC38250.032.6917271
GTGGTCG122350.032.4557452-53
GATCTCG144550.031.39905444-45
TCTCGGT138600.031.29042846-47
TCGGTGG145300.030.82870748-49
GGTCGCC129400.030.74749654-55
TAGATCT149750.030.11563942-43
GTGTAGA164750.029.98495740-41
TCGCCTG156200.029.58711434-35
TGGTCGC122000.029.51199552-53
TCTTCGC148400.028.81963730-31
ATCTCGG168600.028.72325146-47
CTCGGTG162250.028.70596348-49
Produced by FastQC (version 0.11.5)