FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19263721
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT705130.3660403927154053No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG583420.30285945275058745No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC527910.2740436284350256No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA488330.25349723451663364No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG458180.23784605269148157No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG403630.20952857446388473TruSeq Adapter, Index 13 (97% over 37bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG385250.1999873233213874No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT362740.1883021457796238No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT353820.18367168004561527No Hit
GCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCG350110.18174578005983372No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA349730.1815485180666809No Hit
GTGGGTATGATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT327460.16998792704690854No Hit
GTATGATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG323320.1678388095425593No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC291810.15148163742612344No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC283510.14717302020725903No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC270570.1404557302298969No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT259100.13450153269973125No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA255230.13249257503262218No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA246080.1277427138817054No Hit
GGGTATGATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG244880.1271197812717491No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA238150.12362616755091085No Hit
GGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCA234150.12154972551772318No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG219840.1141212541439943No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT210720.10938696630832642No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA201490.10459557631674586No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC198430.10300709816135731No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT194570.10100333159933121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG22400.050.9010122
CGTACGG23750.048.007693
GGCGTAC29200.040.6743241
TATGCCG64900.037.29146-47
TCGTATG67450.036.86822544-45
ATGCCGT81600.030.2703748-49
GCCGTCT84500.029.17442150-51
GTACGGA39950.028.295064
GAGTCTT252200.027.532954
GTATGCC92800.027.18010346-47
TGGAGTC259150.026.948172
GTCGGTT14550.026.7740481
AGTCTTG260550.026.6329775
TACGGAA42350.026.5800325
CGTATGC91650.026.25212744-45
TCTCGTA77650.026.06003442-43
GGAGTCT270650.026.0137733
ATCTCGT76700.025.6717340-41
TGCCGTC98600.024.37688348-49
CGTCTTC105400.023.3892152-53
Produced by FastQC (version 0.11.5)