FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19263721
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT397250.2062166494209504No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA366740.19037858781281145No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC353770.18364572452020045Illumina Single End PCR Primer 1 (96% over 31bp)
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG334480.1736320828151529No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT251040.1303175020028581No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT248740.12912354783377522No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC248120.12880169931863114No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC244610.12697962143450894No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG216780.11253277598860573No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT216490.11238223394119963No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGA214830.11152051049742676No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG212960.1105497738469115No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT197020.10227515234465866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG148500.042.2976652
GTGGCGC170350.037.3195951
CGCCGTA47300.037.06386656-57
CGTATCA53150.035.70569660-61
GGCGCGT189200.033.4049843
GGCGATC30200.033.3792461
GCGATCT54950.030.8921531
CGATCTG57400.029.2195972
CCGTGTA76700.028.54953838-39
TCGCCGT65250.026.83134756-57
GTAGATC87550.026.50080942-43
TCTCGGT81900.026.01186846-47
GCTGCGA139150.025.8666574
GTGGTCG76900.025.57173752-53
GATCTCG87400.025.38066144-45
GCGACAT145500.024.6737147
TCGGTGG90200.024.19811448-49
GAGCCGT87950.024.0856734-35
TAGATCT95500.023.72278842-43
CTCGGTG97550.022.88617148-49
Produced by FastQC (version 0.11.5)