FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19786328
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA485150.24519456060770853No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT400370.20234679218903073No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT361140.18251997035528775No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC360810.18235318852492488Illumina Single End PCR Primer 1 (96% over 31bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC340050.17186109519664286No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT339300.1714820455821818No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG329260.16640783474326312No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG286590.14484243867785876No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC274150.13855526907266472No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC252420.12757293824301305No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT240000.12129587662753796No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG238580.1205782093574917No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG233360.11794002404084275No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT219540.11095540314504035No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC217250.10979803832221927No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT214960.10864067349939817No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG211770.10702844913922382No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT208600.10542633276876842No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG206970.10460253160667304No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT202850.10252028572456699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG155950.043.913032
GTGGCGC173050.039.634441
CGTATCA58600.037.36699360-61
CGCCGTA52000.035.6264456-57
GGCGCGT199850.034.3619353
GCTGCGA147250.031.5425664
GCGACAT149350.031.0354527
GTAGATC90900.030.69874442-43
GGCGATC29350.030.4247441
TGCGACA159250.028.8973376
GATCTCG88800.028.02803844-45
GTGGTCG82900.027.9619352-53
CTGCGAC174850.026.454945
TCTCGGT89550.026.38782346-47
GCGCGTG264350.026.1035654
GGCTGCG181250.025.6780223
TCGCCGT78000.025.4866156-57
GGTCGCC90600.025.24522854-55
TAGATCT99550.025.19238342-43
ATCTCGG110400.024.97521446-47
Produced by FastQC (version 0.11.5)