FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19327789
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA512970.26540542221358066No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC454950.23538646867471494No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC353200.18274206118454625Illumina Single End PCR Primer 1 (96% over 33bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT349370.18076045842594826No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT338680.17522956195351677No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT324710.16800162708729902No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG319010.16505250548834116No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG298550.15446671111734508No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC285550.14774064431270437No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT278430.14405682926277807No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT272390.14093179514739113No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG259450.1342367717280026No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT256740.1328346454941121No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG250740.12973030696889334No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC232650.12037072631535868No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT219870.11375848525664266No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT219810.11372744187139047No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG209250.10826380606700539No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC208650.10795337221448352No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG207890.10756015600128914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG129800.040.9049262
CGTATCA55850.037.25067560-61
GTGGCGC150700.035.646171
GCGACAT143900.035.4777577
CGCCGTA49200.035.43399456-57
GCTGCGA144650.035.0639884
TGCGACA152500.033.2279136
GGCGATC29200.033.1826361
GGCGCGT165300.032.1488723
CTGCGAC165950.030.649315
GGCTGCG174000.029.2586333
GTGGTCG76900.028.3830652-53
GTAGATC94750.028.32345842-43
GGTCGCC77150.028.16915154-55
TCGCCGT67500.027.97386756-57
TAGTGTA96350.027.68311738-39
TCTCGGT83650.027.65264346-47
GATCTCG88250.027.5835444-45
TCGGTGG91050.026.1876748-49
CGACATC197750.025.9847938
Produced by FastQC (version 0.11.5)