FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20195217
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT983780.48713514690136783No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG766200.37939676508551506TruSeq Adapter, Index 19 (97% over 37bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG657390.3255176708425564No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC611310.3027003869282514No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT540170.2674742242185365No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC529870.2623740066769275No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG500060.24761308581135819No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG395550.19586320860033343No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC388130.1921890713033685No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA350330.17347176809241516No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG342220.16945596573683758No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA320120.15851278052620082No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA274730.13603716167050842No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT274390.13586880497496015No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC262160.129812915602739No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC261020.12924842550590074No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA258570.12803526696445006No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC246310.12196452258968053No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG238400.11804775358442547No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA235710.11671575502258777No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG233060.11540356313081458No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA223280.11056083230004411No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC214320.10612413820559591No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT212470.10520807971511274No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT204610.10131606904743831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG16300.045.1653442
CGTACGG17800.041.6261143
TCGTATG115000.040.31318344-45
TATGCCG110150.038.91841546-47
TATCTCG110000.036.44616740-41
GTATGCC130650.035.62944846-47
ATGCCGT130650.035.10562548-49
GCCGTCT131550.034.5771150-51
TCTCGTA116400.034.523142-43
CGTATGC126700.033.9661844-45
ATCTCGT119450.031.03765140-41
GGCGTAC25550.030.303771
TGCCGTC140800.030.2806848-49
CGTCTTC155300.029.42689352-53
GCTATCT142500.028.46644638-39
GAGTCTT353250.028.3720424
CCGTCTT151550.027.96080850-51
TGGAGTC364900.027.750742
GGAGTCT375550.027.064953
AGTCTTG377750.026.8588285
Produced by FastQC (version 0.11.5)