FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19282246
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC487800.25297882829624724Illumina Single End PCR Primer 1 (96% over 31bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT374740.19434457998305799No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA316280.16402653508310183No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT289330.1500499475009291No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG287520.14911126017166257No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG272530.1413372695276266No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT245630.1273866125346601No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT210980.10941671421472374No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG201900.10470771921486739No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC198860.10313113939112695No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC196510.10191240169843284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG154450.039.640582
CGTATCA69000.037.96493560-61
CGCCGTA63250.035.7091656-57
GTAGATC101550.032.85814742-43
GTGGTCG88450.031.6018452-53
GGCGCGT196450.031.2380813
TCTCGGT96550.030.35477446-47
GGTCGCC91250.030.16367554-55
TCGCCGT80650.030.15471556-57
GGCGATC29500.030.1309131
TGGTCGC84050.029.27222652-53
GATCTCG104350.029.1144-45
GCGACAT115950.028.796627
TGCGACA115600.028.7610726
GTGGCGC223250.027.9128321
TAGATCT110200.027.5851342-43
GTGTAGA127500.027.15667240-41
GCTGCGA122100.027.1527354
GTATCAT88950.026.53974260-61
TCGGTGG115200.026.45076848-49
Produced by FastQC (version 0.11.5)