FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17980479
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC399020.22191844833499708Illumina Single End PCR Primer 1 (96% over 33bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC311900.1734659015480066No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT234420.13037472472229467No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA232590.12935695428358723No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG227160.12633701249004545No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC225930.12565293727714374No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG222340.12365632750940617No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC222200.12357846529005151No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT209530.11653193443845405No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT207380.11533619321265023No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG188100.10461345329009311No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG182950.10174923593526067No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC181100.10072034232235971No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA180560.10042001661913456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA60300.040.0160760-61
CGCCGTA55600.037.8898256-57
TGGCGCG65100.037.7155342
TCGCCGT63950.033.98241856-57
GTAGATC94850.032.77551342-43
ATCGGGA26600.032.1405681
TAGTGTA97000.031.4639338-39
TCTCGGT86950.031.13723246-47
GATCTCG93650.030.68464944-45
GTGGTCG82050.030.10396252-53
TCGGTGG91100.030.03162648-49
GGCGCGT82850.029.7534621
CTCGGTG98150.029.71343248-49
TAGATCT98750.029.48495742-43
GGTCGCC84450.029.47374754-55
GCTATAG95550.028.86261434-35
GTGTAGA109000.028.39021740-41
TGGTCGC79050.028.21191852-53
TATAGTG108950.027.95045536-37
GTATCAT82100.027.04746260-61
Produced by FastQC (version 0.11.5)