FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16665076
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC408690.2452374054579769Illumina Single End PCR Primer 1 (96% over 31bp)
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA340770.20448151571585993No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT250070.15005632137531205No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG221750.13306269950404068No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC213810.1282982447844822No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT186300.11179066930147813No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT180920.10856236119175214No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC174540.10473399581256036No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA172880.10373790074524714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG89150.039.154592
CGTATCA54650.037.9385360-61
CGCCGTA47950.037.89216656-57
CCGTGTA75650.033.15441538-39
TCGCCGT56650.033.03717856-57
GTGGCGC107600.032.9748151
GCGACAT107200.032.163157
GTGGTCG71600.030.98095952-53
GCTGCGA111850.030.826014
TGCGACA111700.030.6973386
GGCGCGT114350.030.6089213
GTAGATC91250.030.55506142-43
TCTCGGT78150.030.51064546-47
GGTCGCC72250.030.40748654-55
TGGTCGC67150.029.7801652-53
TCGGTGG84350.029.1971448-49
GGCGATC22850.028.8936631
GATCTCG89900.028.68887344-45
CTGCGAC121450.028.467585
CTCGGTG91250.028.21259748-49
Produced by FastQC (version 0.11.5)