FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15896253
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA358650.22561920724336734No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC290770.18291732020118198No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC284420.17892266812814314Illumina Single End PCR Primer 1 (96% over 33bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT283890.1785892562228344No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG232720.14639928038387412No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT231530.14565067629459597No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT221380.1392655237684No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC200290.12599824625337808No Hit
CTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAG199420.12545094746541843No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG191980.12077059920976346No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG175300.110277560378537No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT164370.10340172618037721No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT160720.10110558758721316No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC159220.10016196898728273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG102900.040.4305232
CGCCGTA41450.037.53140356-57
CGTATCA50000.036.47897760-61
GCGACAT104550.035.2954677
TGCGACA106050.034.7066766
GCTGCGA107900.034.2436564
GTGGCGC127000.032.9867941
TCGCCGT51000.032.92511456-57
GGCGCGT130150.032.001923
GTGGTCG60800.031.28931452-53
CTGCGAC117650.031.284685
TCTCGGT68200.029.87748546-47
GGTCGCC64800.029.35861454-55
GTAGATC79750.029.30265242-43
TCGGTGG71000.029.30130248-49
ATCGGGA27200.029.162371
TGGTCGC58100.028.90083552-53
GGCGATC20050.028.1908281
GGCTGCG132250.027.866773
GATCTCG78250.027.61820644-45
Produced by FastQC (version 0.11.5)