FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13607219
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC287970.21163031182198214Illumina Single End PCR Primer 1 (96% over 33bp)
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC219600.16138492369381283No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC207300.15234560419730145No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA205570.15107422023559702No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG202250.14863433887556304No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG199340.1464957681654128No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC185420.13626590414984868No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC174380.12815256372371164No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA167150.12283920762942081No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT161030.11834159500188834No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA141200.10376844820385416No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA137780.10125507644140952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA44950.038.68135560-61
CGCCGTA40050.036.3599256-57
ATCGGGA17050.032.878071
GTGGTCG53400.032.47218352-53
TGCGACA41050.031.8132936
TCGCCGT49400.031.25729656-57
TGGTCGC48500.031.24703652-53
GGTCGCC54850.031.22652254-55
TGGCGCG44000.031.0846822
GCGACAT42250.030.7986767
TCTCGGT60500.030.22721946-47
GCTGCGA42900.030.2198894
GTAGATC71350.029.95657742-43
TAGTGTA76950.028.73335538-39
TCGGTGG68250.028.11716548-49
GATCTCG69100.028.00993344-45
CTGCGAC48200.027.1930245
TATAGTG82150.026.91456636-37
GTGTAGA84050.026.47626740-41
GTATCAT60450.026.24825560-61
Produced by FastQC (version 0.11.5)