FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17692557
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC399910.22603290185810904Illumina Single End PCR Primer 1 (96% over 31bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT365790.20674795621684305No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG323040.18258525322258393No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT315820.1785044411613313No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG313400.17713663434855684No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA294710.1665728701622948No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT294370.16638069895719426No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG279880.15819081436335064No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC276400.15622388555820393No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC229510.12972121553713237No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC227850.1287829678887003No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT203710.11513881232656197No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC201570.11392926415328208No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG200620.11339231519785412No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG199820.11294014765644107No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT196340.11097321885129435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG156900.043.8288152
CGCCGTA53500.037.91295656-57
CGTATCA60400.036.37135760-61
GCCGTAT63400.034.57544358-59
CCGTATC62450.033.23795758-59
GGCGCGT207400.033.1343
GTGGCGC211350.032.879291
GTAGATC94350.029.9538342-43
TCTCGGT87600.029.3066346-47
GGCGATC26650.029.0516471
TGGTCGC75150.029.04425652-53
GTGGTCG81100.028.75837752-53
ATCGGGA28600.028.7316461
ATCTCGG97500.028.2795146-47
GATCTCG95000.028.24870144-45
TCGCCGT71600.027.93075256-57
TAGTGTA102000.027.89600638-39
GCGCGTG254650.027.0046774
TCGGTGG98650.026.938748-49
GGTCGCC85300.026.8139554-55
Produced by FastQC (version 0.11.5)