FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18854128
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT1913471.014881197369616No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG1273140.6752579594240582No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT1106360.5867998774591962No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC1014860.5382693911911492No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA991370.5258105811098769No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG956110.5071091062922666No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG931530.4940721734784022No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG838630.4447991442510627No Hit
TGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGC820960.43542719132913493No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT753180.3994775043428155No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCC716300.3799168012437383No Hit
GTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGA706460.37469778501556794No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA666690.35360426109337967No Hit
GCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCG658640.34933463907744766No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC654600.34719187225206066No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG651300.34544159241944256No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG643180.34113484325554594TruSeq Adapter, Index 3 (97% over 35bp)
CGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTG608040.32249701497730365No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGG599550.31799402231702256No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT590800.3133531288214443No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA565170.2997592887881105No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA546230.2897137433245388No Hit
CGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCT543860.28845672417202217No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCG541430.28716788174982155No Hit
GGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCG496540.2633587721479349No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC483530.25645842650479517No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA475190.252034992018724No Hit
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAG455800.24175077203252254No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATG446040.2365741868305975No Hit
GTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGG442980.2349512000767153No Hit
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGA435130.2307876556263965No Hit
CTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAA426370.2261414582525376No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA423240.22448134435069075No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA419320.2224022240646717No Hit
CGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGC418110.2217604547927117No Hit
GTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTC413070.21908730013925864No Hit
GTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCC411170.21807956326593303No Hit
GTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGT403290.21390010718077232No Hit
GTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGC397330.21073899572549842No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCG384500.20393411989141053No Hit
TGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTG376190.1995265970401813No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT370740.19663598337722113No Hit
GCCGAAGTGGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTCAGT364340.1932415012775982No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA363390.19273763284093542No Hit
GTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGC361140.19154426022778673No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCTC355980.1888074590349657No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA341120.18092589590990366No Hit
GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG339130.17987042413205215No Hit
GTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGA334440.1773829052184222No Hit
TCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGG331710.17593494644780178No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT330150.17510754143601867No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCA328490.17422709764142896No Hit
GTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAG325700.1727473156011246No Hit
CGAAGTGGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCG325280.17252455271333683No Hit
GTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTG315740.16746465283358636No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA311820.1653855325475673No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCA310710.16479680205841396No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGT307240.1629563562950246No Hit
GTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCG306670.16265403523302693No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT301660.15999679221441587No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT296770.15740319573517267No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTC293010.1554089375016442No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC292870.15533468320571495No Hit
CGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCC291840.15478838374280687No Hit
GCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCAT286200.15179699639251415No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG285740.15155301799160376No Hit
GGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGA282910.15005202043817673No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG276900.14686438959149953No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT274760.14572935963943812No Hit
TCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCG273990.14532096101182723No Hit
GTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTC271750.1441328922769592No Hit
CTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCAT271650.1440798534941526No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT268190.14224471160904392No Hit
GTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAAC267350.1417991858334684No Hit
GTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCG266390.14129001351852497No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC265790.1409717808216853No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGA264340.14020271847098947No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG264330.14019741459270882No Hit
CAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGG256190.1358800576722509No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT252720.13403961190886154No Hit
GCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTT252720.13403961190886154No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG251530.13340845039346289No Hit
GTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAA251190.13322811853192046No Hit
GGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGA250850.13304778667037798No Hit
GCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCG250450.13283563153915154No Hit
GGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGG247960.13151496584726696No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA247030.1310217051671655No Hit
CTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCC245140.13001927217212061No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTG244520.12969043171871963No Hit
GGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCG243180.12897971202911107No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGG243080.12892667324630447No Hit
TGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA242680.12871451811507803No Hit
CTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTT238640.12657175128969106No Hit
TGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCC234080.12415318279370968No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC233840.12402588971497383No Hit
CCGAAGTGGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTCAGTC229660.12180886859365758No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG225960.11984643362981307No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT222900.11822344687593084No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG220930.11717858285464064No Hit
AGAAAAGGTGTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCG220460.11692930057544956No Hit
CGCCGCGCCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGA220330.11686035015780098No Hit
GTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAA220180.11678079198359106No Hit
TGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTTG219760.11655802909580332No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG219580.11646255928675142No Hit
GGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA218200.11573062408402023No Hit
CGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC217600.11541239138718057No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG216980.11508355093377959No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAG214920.11399095200796344No Hit
GTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCG214650.11384774729438561No Hit
GGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGG213940.11347117193645868No Hit
GGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT212030.11245813118485246No Hit
GCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCC209260.11098895690110941No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC209130.1109200064834608No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG207130.10985923082732862No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA206910.1097425455051541No Hit
CGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATG202820.10757325928836381No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTG202670.10749370111415388No Hit
TCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCT202150.10721789944355953No Hit
GAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGG200900.10655491465847691No Hit
CCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCG200060.1061093888829014No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG199850.10599800743900752No Hit
GTCTCGCCCGCCGCGCCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGAC197870.10494783953943668No Hit
GGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGG197300.104645518477439No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA197210.10459778357291305No Hit
AGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTC196320.10412573840593424No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCC195820.1038605444919012No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC193430.10259291758282325No Hit
GGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTC193390.1025717020697006No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCT190030.10078959896739856No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCC189460.10048727790540088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA12200.063.0761871
TCGAAAC10850.062.1623462
GGCGTAC54100.055.316351
GCGTACG55550.053.8673062
CGTACGG57200.052.3964773
CGGGTTT14250.047.3351481
GATCGTT205300.045.6260835
ATCGTTT208900.044.95356
CTCTGAT213200.044.6499251
TCGTTTT211650.044.41437
GTACGGA109350.043.8745651
TGATCGT216900.043.251664
TACGGAA111900.042.7857552
TCGTATG93850.041.9334344-45
CGTTTTT225700.041.6494678
TATGCCG92150.041.2633746-47
GCCGTCT95400.040.85595750-51
GTATGCC97100.040.62740746-47
ATGCCGT96600.040.42203548-49
CGTATGC95900.039.60062444-45
Produced by FastQC (version 0.11.5)