FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16912314
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG766360.4531372821010774TruSeq Adapter, Index 7 (97% over 35bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT670790.3966281609955918No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG631820.37358577897737705No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC622030.3677970974285364No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC473690.280085859333028No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT459420.27164822034406405No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG402880.23821695836536622No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG377860.22342300409039237No Hit
GTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG370340.21897653981589982No Hit
GTGGGTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT331380.195940070649114No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA313790.1855393649857731No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT299150.17688295049394187No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA267890.15839937692736783No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA265110.1567556042301485No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC257390.15219088292707905No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT247680.14644950418966912No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC244000.14427357486385364No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT221240.1308159250117991No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGAAATTTGAAGTAGATAGAAA215550.12745151254878545No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA214560.12686614025732967No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG210400.12440639406292953No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG210170.12427039848006607No Hit
GGGTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG208760.12343668642859872No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC203600.12038565509131394No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC202890.11996584263986584No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC192730.11395838558815782No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG183250.1083530024336114No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA179370.10605881607921895No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATGCTAAGTTGAGATGATATCATTT177220.10478755302201698No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA169760.1003765658560975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG20400.049.1286472
CGTACGG21200.046.6025853
TCGTATG116600.041.9789944-45
TATGCCG114900.040.28508846-47
GTATGCC129250.037.9255346-47
GCCGTCT128750.037.87001850-51
GGCGTAC27000.037.64731
ATGCCGT130450.037.28536248-49
TCTCGTA111250.036.86759642-43
CGGCTAT131800.035.48119732-33
CCGGCTA140750.035.03054432-33
CGTATGC132950.034.85149844-45
GTACGGA41300.034.0428161
ATCTCGT113050.033.8856940-41
GATCGTT46300.033.649655
TGCCGTC138150.033.48810648-49
TACGGAA42350.032.6378942
CGTCTTC149100.032.5738852-53
GGCTATG152700.032.47547534-35
ACCGGCT146150.032.07885430-31
Produced by FastQC (version 0.11.5)