FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16912314
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC648480.3834365894578353Illumina Single End PCR Primer 1 (96% over 31bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT340060.20107242568935274No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA332890.19683291121486984No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG306520.1812407219970017No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC281870.16666554322489519No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT256750.15181246043563287No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG231890.1371131117835206No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC230300.136172968406334No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT192200.11364500446242898No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT187070.11061171167943074No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT182410.10785632291358828No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG181280.10718817070212863No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT172540.10202033855331682No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG171980.10168921887330144No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC171010.1011156722847033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA86500.041.4591960-61
TGGCGCG126500.041.038012
CGCCGTA78350.039.8914256-57
ATCGGGA31750.039.3743061
TCGCCGT89000.036.8524456-57
GTACGTG120150.036.2689636-37
GTGGTCG102550.036.21816352-53
GTAGATC125050.035.3244842-43
ACGTGTA124850.035.11278538-39
TGGTCGC95100.034.96011452-53
CAGTACG123400.034.91089234-35
GTGGCGC150500.034.6790121
TACGTGT116350.034.6572536-37
GGTCGCC106150.034.65427454-55
TCTCGGT115650.034.2344146-47
TCGGTGG120600.034.0699648-49
GATCTCG121900.033.92094444-45
AGTACGT119700.033.82743534-35
CTCGGTG132300.032.4751848-49
GGCGCGT164700.031.404433
Produced by FastQC (version 0.11.5)