FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20427007
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG846810.4145541243511592TruSeq Adapter, Index 7 (97% over 35bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT657700.321975705985708No Hit
GTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG464990.2276349148947763No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC427360.2092132244337117No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG426440.20876284029275557No Hit
GTGGGTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT400980.19629894873977377No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT358140.1753267133065554No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA340100.1664952677599807No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT330430.16176133880014826No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC322220.15774214989009405No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT316220.15480486201429314No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT284760.13940368258551045No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG275400.13482151349926105No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGAAATTTGAAGTAGATAGAAA259970.12726778817865975No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG256220.12543198325628419No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG254180.1244333053785119No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA241210.11808386808698895No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA238200.11661032866929551No Hit
GGGTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG238010.11651731455322849No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG237470.11625295864440639No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC234300.11470109155002493No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT230350.11276737703178932No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG220480.10793553847609687No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC218250.10684384648225753No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG214370.10494440032257296No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC213440.10448912070182381No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA205570.10063637810473164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG22250.043.549612
TATGCCG121650.041.54450646-47
CGTACGG23500.041.233143
TCGTATG127600.040.8729344-45
ATGCCGT138700.037.44781548-49
GTATGCC139850.037.3606446-47
GATCGTT63450.036.7565775
GCCGTCT141650.036.75184650-51
CGTATGC139200.036.27255644-45
TCTCGTA122750.035.8322142-43
CGGCTAT143950.035.5882332-33
TGCCGTC149350.034.0459648-49
ATCTCGT126100.033.82567240-41
CCGGCTA156900.033.64995232-33
GGCGTAC30050.032.4037781
ACCGGCT159600.032.2178230-31
ATCGTTT73100.032.034266
GTACGGA43900.031.37761
TCACCGG165900.031.00821928-29
CGTCTTC170950.030.633452-53
Produced by FastQC (version 0.11.5)