FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20427007
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC711880.3484994155041901Illumina Single End PCR Primer 1 (96% over 32bp)
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA324170.15869676844972933No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC323500.15836877130359822No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG227000.11112739130113383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA84450.041.34108456-57
CGTATCA93700.040.55481360-61
TCGCCGT97700.036.05045356-57
TGGTCGC104800.035.486952-53
GTGGTCG113400.035.12024752-53
GGTCGCC115550.034.4256754-55
TCTCGGT126950.034.29225546-47
TCGGTGG131100.034.0944148-49
GTAGATC141050.034.0789442-43
GTACGTC133150.033.5503430-31
GATCTCG141650.032.4437244-45
ATCGGGA35200.032.410761
CTCGGTG145800.031.34106848-49
TGGCGCG81300.030.9060382
GTGTACG149800.030.70909728-29
GCGACAT106450.030.6974167
TGCGACA105700.030.6912336
TAGATCT153050.030.4138542-43
TACGTCC141650.030.31315430-31
ACGTCCT149050.029.92353432-33
Produced by FastQC (version 0.11.5)