FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003952731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21449875
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC393860.1836187856572591Illumina Single End PCR Primer 1 (96% over 31bp)
CCCAAACCCACTCCACCCTACTACCAGACAACCTTAGCCAAACCATTTAC346080.16134359757341243No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA315990.14731554379687528No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT258600.12056014312437717No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT256790.11971631536314313No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG249990.11654613371872796No Hit
CTCCACCCTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG249000.11608459256755574No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT239870.11182815750674538No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT231500.10792603686501669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)