FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17783833
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA303870.1708686760610044No Hit
CCCAAACCCACTCCACCCTACTACCAGACAACCTTAGCCAAACCATTTAC291000.16363176599780263No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC250570.1408976343851182Illumina Single End PCR Primer 1 (96% over 33bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT210860.11856836487387167No Hit
CTCCACCCTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG201830.11349071935167182No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG197520.11106716982778683No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT190560.10715350284722085No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT188420.10595016271239165No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA183310.10307676640913127No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT183280.1030598971549047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG77600.036.84792
CGCCGTA35700.035.1248856-57
CGTATCA41600.035.11001260-61
GCGACAT101300.031.1795187
TGCGACA104750.030.1077696
GGCGATC22450.029.6404821
GCTGCGA108400.029.2263454
GTAGATC65950.028.16082842-43
TCGCCGT48300.027.7811756-57
GTGGCGC105250.027.5474431
CTGCGAC117400.026.985375
GTGGTCG57300.026.9809552-53
TCTCGGT62450.026.58410346-47
GATCTCG65950.026.32569544-45
TGGTCGC52800.025.90713952-53
GGCGCGT110250.025.8924923
TCGGTGG68400.025.17440448-49
GGGTGTA68450.025.08393938-39
GGTCGCC61450.024.69504554-55
TAGGGTG70500.023.98400736-37
Produced by FastQC (version 0.11.5)