FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17852265
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC529580.29664583177540776Illumina Single End PCR Primer 1 (96% over 31bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT256440.14364563824254234No Hit
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGC230950.12936733798204317No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG222630.12470686492722352No Hit
GCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAAC213480.1195814648729447No Hit
GCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGG197320.11052939220877575No Hit
GCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCG194800.10911780662005634No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA186660.10455816110728808No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG181420.10162295932756991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG69800.046.0819242
CGTATCA72300.040.3738960-61
CGCCGTA66500.039.14946756-57
GTCTAAC38600.039.043771
ATCGGGA23850.036.8776441
GTAGATC99050.035.29310642-43
TCGCCGT77650.034.6903456-57
GATCTCG100450.033.4562444-45
TAGATCT101150.033.1281942-43
ATCATTA98300.032.8090162-63
TCGGTGG101250.032.7699948-49
CCGTGTA102450.032.0367538-39
GGTCGCC94050.031.7730454-55
TATCATT96550.030.57495162-63
TGGTCGC91300.030.33058252-53
GTGTAGA114150.030.12662740-41
GAGCCGT106350.029.63195434-35
TCATTAA118500.029.14022664-65
AGCCGTG116000.028.66240936-37
GGCGCGT113200.028.5673073
Produced by FastQC (version 0.11.5)