FastQCFastQC Report
Thu 23 Mar 2023
EGAF00004041445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004041445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94542569
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG11690481.2365308160813782TruSeq Adapter, Index 5 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1616220.1709515636284434No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1298900.1373878469496635TruSeq Adapter, Index 5 (98% over 63bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)