FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004043153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11190792
Sequences flagged as poor quality0
Sequence length13-34
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTGGTTATCTAGCTGTATGA8926227.976397023552935No Hit
TCCCTGAGACCCTAACTTGTGA8291227.408966228663709No Hit
TCTTTGGTTATCTAGCTGTATG1903231.7007107271764144No Hit
AACATTCAACGCTGTCGGTGAGT1855501.65805959042041No Hit
TCTTTGGTTATCTAGCTGTAT1521901.3599573649478964No Hit
AACCCGTAGATCCGATCTTGTG1126441.0065775505433396No Hit
TGAGGTAGTAGGTTGTATAGTT902730.8066721282997664No Hit
TGAGGTAGTAGGTTGTATGGTT831260.7428071221411318No Hit
AACATTCAACGCTGTCGGTGAGTT821770.7343269359308975No Hit
TTCAAGTAATCCAGGATAGGCT810620.7243633873277244No Hit
TCCCTGAGACCCTAACTTGTGAA772110.689951166995151No Hit
AACCCGTAGATCCGATCTTGT704000.6290886292945128No Hit
TAGCTTATCAGACTGATGTTGA698340.6240308996896734No Hit
TCTTTGGTTATCTAGCTGTATGT667860.5967942215349906No Hit
TATTGCACTTGTCCCGGCCTGT638040.5701473139702712No Hit
CAAAGTGCTGTTCGTGCAGGTAG612370.547208812387899No Hit
TAGCTTATCAGACTGATGTTGAC604090.5398098722592646No Hit
CAGTGCAATGTTAAAAGGGCAT589530.5267991756079462No Hit
TCCCTGAGACCCTAACTTGTG565000.5048793686809655No Hit
TACAGTACTGTGATAACTGAAG544320.4863998901954393No Hit
CATTGCACTTGTCTCGGTCTGA530380.4739432204619655No Hit
TGAGGTAGTAGATTGTATAGTT527210.4711105344465343No Hit
AACCCGTAGATCCGATCTTGTGA502810.44930689445394034No Hit
CTTTTTGCGGTCTGGGCTTGC483140.4317299436894189No Hit
TAAGGCACGCGGTGAATGCCA480120.42903129644443394No Hit
AACATTCAACGCTGTCGGTGAGTTT467700.41793288625148245No Hit
AACCCGTAGATCCGAACTTGTG460220.41124881956522824No Hit
CTTTGGTTATCTAGCTGTATGA440770.3938684589973614No Hit
AACCCGTAGATCCGATCTTGTGT438040.3914289533752392No Hit
TGTGCAAATCCATGCAAAACTGA436110.38970432119549714No Hit
TCAGTGCACTACAGAACTTTGT420890.37610385395421525No Hit
TAAGGCACGCGGTGAATGCC365750.3268312019381649No Hit
TATACAAGGGCAAGCTCTCTGT364290.32552655790582113No Hit
TATGGCTTTTTATTCCTATGTGA359180.32096030379261803No Hit
GTACAGTACTGTGATAACTGA353400.3157953431714216No Hit
TACCCTGTAGAACCGAATTTGT337260.30137277147140257No Hit
TGTAAACATCCTTGACTGGAAGCT331280.2960290924896111No Hit
TCCCTGAGACCCTAACTTGT316510.282830741559668No Hit
ATCTGGAGGTAAGAAGCACTTTCT309910.2769330356600319No Hit
AACATTCAACGCTGTCGGTGAG297980.2662724854505383No Hit
TCCCTGAGACCCTAACTTGTGAAA294090.2627964133369649No Hit
TGTAAACATCCTACACTCTCAGCT291430.26041945914105097No Hit
TTCACAGTGGCTAAGTTCTGC290750.25981181671502784No Hit
TGTAAACATCCCCGACTGGAAGCT286920.256389360109633No Hit
TAAAGTGCTGACAGTGCAGATA282720.25263627453713733No Hit
CAAGTGCTTCCTTTTAGAGGGT269330.2406710802952999No Hit
TATTGCACTCGTCCCGGCCTCC268090.23956302645961072No Hit
AGAATTGTGGCTGGACATCTGT253710.2267131763328279No Hit
TTCTCCAAAAGAAAGCACTTTCT253610.2266238171525304No Hit
AAGTGCTGTCATAGCTGAGGTC245280.21918019743374734No Hit
AACATTCATTGCTGTCGGTGGGTT235450.2103961900105015No Hit
AGCAGCATTGTACAGGGCTATGA234310.2093774953551098No Hit
TCAGTGCATCACAGAACTTTGT233370.20853751906031318No Hit
TGAGGTAGTAGTTTGTACAGTT231930.20725074686402892No Hit
TAAGGCACGCGGTGAATGC230750.20619630853651824No Hit
AACCCGTAGATCCGAACTTGT225480.20148707973483915No Hit
TGAGGTAGTAGTTTGTGCTGTT223810.19999478142387062No Hit
TCGGATCCGTCTGAGCTTGGCT223270.19951224185026403No Hit
AACATTCAACCTGTCGGTGAGTTT219760.1963757346218212No Hit
TGGAAGACTAGTGATTTTGTTGTT215420.19249754619690904No Hit
AAATCTCTGCAGGCAAATGTGA213950.19118396624653555No Hit
CAAAGTGCTTACAGTGCAGGTAG212370.1897720911978348No Hit
TGTAAACATCCTCGACTGGAAGCT195830.17499208277662565No Hit
TGTAAACATCCCCGACTGGAAGC193920.1732853224329431No Hit
AAGTGCTGTCATAGCTGAGGTCT190810.17050625192569033No Hit
TGAGGTAGTAGGTTGTATAGT190120.16988967358163748No Hit
TGAGATGAAGCACTGTAGCTCT189850.16964840379483417No Hit
TTCAAGTAATTCAGGATAGGTT188970.16886204300821606No Hit
GGTAGCGTGGCCGAGC187750.16777186100858635No Hit
GGGTGCTGTAGGCTTT186500.16665487125486741No Hit
TTTGGCAATGGTAGAACTCACACT185310.16559149700932696No Hit
TCACAGTGAACCGGTCTCTTT180130.16096269146991563No Hit
TGTAAACATCCTACACTCTCAGC179120.16006016374891072No Hit
ATAAAGCTAGATAACCGAAAGT175410.15674493815987287No Hit
TCCCTGAGACCCTAACTTGTGT173330.15488626720968454No Hit
AGCTCGGTCTGAGGCCCCTCAGT172280.15394799581656063No Hit
AACCCGTAGATCCGAACTTGTGA169820.1517497599812417No Hit
TCGAGGAGCTCACAGTCTAGT166860.14910472824443524No Hit
GTCTCTGTGGCGCAATGGAC165480.14787157155632952No Hit
TAAAGTGCTGACAGTGCAGAT165440.1478358278842105No Hit
ATCGTGCATCCCTTTAGAGTGT160160.1431176631645017No Hit
AACATTCAACCTGTCGGTGAGTT159470.14250108482044882No Hit
CTCTTGAGGGAAGCACTTTCTGT158620.14174153178791993No Hit
GGGTGCTGTAGGCTT155320.1387926788381019No Hit
TCCCTGAGACCCTTTAACCTGT155210.13869438373977463No Hit
GGTGCTGTAGGCTTT154380.13795270254330524No Hit
TGTAAACATCCTTGACTGGAAGC149970.1340119626921848No Hit
CGTACGCGGGTTTAAACGA149690.13376175698735174No Hit
CGGGTGCTGTAGGCTTT147700.13198350929943117No Hit
TAAAGTGCTTATAGTGCAGGTAG147590.1318852142011039No Hit
TAGCAGCACGTAAATATTGGCG146660.131054173824337No Hit
CAACGGAATCCCAAAAGCAGCT144320.12896316900537513No Hit
TATGGCACTGGTAGAATTCACT144230.12888274574310737No Hit
AACATTCATTGCTGTCGGTGGGT144170.12882913023492887No Hit
TCGTACCGTGAGTAATAATGCG142550.12738151151410912No Hit
TACTGCATCAGGAACTGATTGGA141330.1262913295144794No Hit
AACATTCAACGCTGTCGGTGA140480.12553177648195052No Hit
CGGGTGCTGTAGGCTT139310.12448627407246958No Hit
TTCTCGAGGAAAGAAGCACTTT139020.12422713244960679No Hit
TGTGCAAATCCATGCAAAACTG135650.1212157280735805No Hit
TGTAAACATCCTCGACTGGAAGC135180.12079573992618217No Hit
TGAGGTAGTAGGTTGTGTGGTT132330.11824900328770296No Hit
TGTAAACATCCTACACTCAGCT132210.11814177227134594No Hit
TCTGGCTCCGTGTCTTCACTCCC129060.1153269580919742No Hit
AAGCCCTTACCCCAAAAAGCAT128320.11466570015777257No Hit
TCAAAACTGAGGGGCATTTTCT127070.11354871040405361No Hit
TACAGTACTGTGATAACTGA126080.11266405451910821No Hit
TTAAGGCACGCGGTGAATGCCA125470.11211896351929337No Hit
ATCTGGAGGTAAGAAGCACTTT125350.11201173250293633No Hit
TGAGATGAAGCACTGTAGCTC123550.11040326725758107No Hit
TTTGGCACTAGCACATTTTTGCT123440.11030497215925379No Hit
CCGGGTGCTGTAGGCTTT122130.10913436689735632No Hit
TCCCTGAGACCCTTTAACCTGTG121480.10855353222542247No Hit
CACCCGTAGAACCGACCTTGCG117680.10515788337411686No Hit
TAAAGCTAGATAACCGAAAGTA116850.1044162021776475No Hit
AAGTGCTGTCATAGCTGAGGTCA115920.10358516180088058No Hit
TTCTCGAGGAAAGAAGCACTTTC115250.10298645529288723No Hit
TGAGATGAAGCACTGTAGCT114930.10270050591593517No Hit
CCGGGTGCTGTAGGCTT114830.10261114673563766No Hit
TTCAAGTAATCCAGGATAGGCTT113230.1011813998508774No Hit
TGAGGTAGGAGGTTGTATAGTT112860.10085077088377659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)