FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004043154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26271538
Sequences flagged as poor quality0
Sequence length13-34
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTGGTTATCTAGCTGTATGA6621722.520491948358714No Hit
TATTGCACTTGTCCCGGCCTGT4652291.770847980045934No Hit
TACCCTGTAGAACCGAATTTGT4153831.5811141319552742No Hit
TAGCTTATCAGACTGATGTTGA4149301.5793898324490938No Hit
TCCCTGAGACCCTAACTTGTGA3913841.4897643221344712No Hit
TAGCTTATCAGACTGATGTTGAC3429341.3053442093873606No Hit
TGAGGTAGTAGGTTGTATAGTT2737351.0419450890161057No Hit
AAGTGCTGTCATAGCTGAGGTC1968260.7491986194337005No Hit
TCTTTGGTTATCTAGCTGTAT1753070.667288683289117No Hit
TCTTTGGTTATCTAGCTGTATG1651200.628512879603775No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC1638920.623838619573776No Hit
ATCGTGCATCCCTTTAGAGTGT1555240.591986658717887No Hit
CAAGTGCTTCCTTTTAGAGGGT1470100.5595789633633174No Hit
TCACAGTGAACCGGTCTCTTT1406720.5354539958794952No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG1401380.5334213779185673No Hit
TATTGCACTCGTCCCGGCCTCC1321850.5031490733431746No Hit
AGAATTGTGGCTGGACATCTGT1226020.46667233566607336No Hit
AACATTCAACGCTGTCGGTGAGT1177280.4481199387717613No Hit
TGAGGTAGTAGATTGTATAGTT1110100.42254853903109896No Hit
AAGTGCTGTCATAGCTGAGGTCT1008050.38370422013359096No Hit
CTCTTGAGGGAAGCACTTTCTGT992930.3779489423116378No Hit
CATTGCACTTGTCTCGGTCTGA953070.36277662921752046No Hit
CAAAGTGCTGTTCGTGCAGGTAG921700.350835950297238No Hit
AAGTGCTGTCATAGCTGAGGTCA886730.33752496713363334No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG879990.33495945307808017No Hit
TACCCTGTAGAACCGAATTTGTG860950.32771206619117615No Hit
AAGTGCTGTCATAGCTGAGGT855150.3255043537991571No Hit
TGAGGTAGTAGGTTGTGTGGTT807540.3073820801812212No Hit
TTCAAGTAATCCAGGATAGGCT787620.29979973003483845No Hit
TCAAAACTGAGGGGCATTTTCT765620.29142564854786956No Hit
TGTGCAAATCCATGCAAAACTGA761760.28995637788697415No Hit
GGGTGCTGTAGGCTTT756620.2879998879395641No Hit
CTACAAAGGGAAGCCCTTTCT731910.2785942718694277No Hit
TGAGGTAGTAGGTTGTATGGTT729200.27756273728626013No Hit
CGGGTGCTGTAGGCTTT712890.27135449778387544No Hit
AAGTGCTGTCATAGCTGAGG693180.26385208205168653No Hit
CAAAGTGCCTCCCTTTAGAGTGT685570.26095541113733045No Hit
AACCCGTAGATCCGATCTTGT681280.2593224652473715No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG677490.25787983939120734No Hit
TAGCACCATCTGAAATCGGTTA653920.2489081529981229No Hit
GCCGCCGGTGAAATACCACTACTCTGA651660.24804790644537064No Hit
ACCGGGTGCTGTAGGCTTT646120.24593916047092484No Hit
TGAGGTAGTAGGTTGTATAGT622210.23683805645486No Hit
TTCTCCAAAAGAAAGCACTTTCT614360.23385003192428247No Hit
CCGGGTGCTGTAGGCTTT605710.23055749533963332No Hit
CAGTGCAATGTTAAAAGGGCAT605680.23054607613760564No Hit
TACCCTGTAGATCCGAATTTGT562260.21401868440286978No Hit
TAGCACCATCTGAAATCGGTT560900.21350101391094806No Hit
CGGGTGCTGTAGGCTT558400.2125494137419743No Hit
TGAGATGAAGCACTGTAGCTCT547750.20849559702214615No Hit
TTCTCGAGGAAAGAAGCACTTTC546030.20784089610589224No Hit
AAAGTGCATCCTTTTAGAGTGT538540.20498990199964692No Hit
CTACAAAGGGAAGCACTTTCTC537290.20451410191516003No Hit
ACCGGGTGCTGTAGGCTT523880.19940971860878492No Hit
GCCGCCGGTGAAATACCACTACT522990.19907094894863026No Hit
CTCCAGAGGGAAGTACTTTCT505090.1922574917387783No Hit
TAAGGCACGCGGTGAATGCCA503170.19152666280900646No Hit
CAAGTGCTTCCTTTTAGAGGGTT495070.18844347826153157No Hit
TATTGCACTTGTCCCGGCCTGTA484090.18426405031939888No Hit
AGCTACATTGTCTGCTGGGTTTC477530.1817670514760118No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTC472700.17992855994955453No Hit
TAGCTTATCAGACTGATGTTG472340.17979152952522232No Hit
ATCGTGCATCCTTTTAGAGTGT468840.1784592892886591No Hit
CCGGGTGCTGTAGGCTT457720.17422657173706388No Hit
ATCTGGAGGTAAGAAGCACTTTCT451670.17192369932814744No Hit
TCTTTGGTTATCTAGCTGTATGT443480.16880625717458947No Hit
TCACAGTGAACCGGTCTCTTTT440960.16784704420426394No Hit
TGAGGTAGTAGTTTGTACAGTT439140.16715427928125107No Hit
AACCCGTAGATCCGATCTTGTG437190.16641203114945155No Hit
AAAGTGCTTCCCTTTGGACTGT437090.1663739671426926No Hit
TATTGCACTCGTCCCGGCCTC422180.1606986237349332No Hit
TGAGGTAGTAGTTTGTGCTGTT422090.16066436612885016No Hit
AAAGTGCTTCCTTTTAGAGGGT412730.15710157509621248No Hit
CTCTAGAGGGAAGCGCTTTCT403410.15355400966627838No Hit
TAAAGTGCTTATAGTGCAGGTAG398290.15160513252022018No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCG395200.15042895471136863No Hit
TTCTCGAGGAAAGAAGCACTTTCT388770.14798143907676817No Hit
ACTCAGCCTTGAGGGCACTTTC382840.14572424347596247No Hit
AGCTACATTGTCTGCTGGGTTT377330.14362691670354436No Hit
ACGCGACCTCAGATCAGACGT375030.1427514445480885No Hit
GGGTGCTGTAGGCTT374680.14261822052443218No Hit
TGTAAACATCCTTGACTGGAAGCT372660.1418493275879014No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT366420.13947413356614297No Hit
TCCCTGAGACCCTTTAACCTGT359640.13689339390788616No Hit
CAAAGCGCTCCCCTTTAGAGGT357950.13625011219365993No Hit
TGAGATGAAGCACTGTAGCTC354370.13488742075168952No Hit
TATTGCACTCGTCCCGGCCT345610.13155301375960554No Hit
TAAAGTGCTGACAGTGCAGATA342900.130521479176438No Hit
CAAAGTGCTTACAGTGCAGGTAG342680.13043773836156833No Hit
TAACAGTCTACAGCCATGGTCG336880.12823002596954924No Hit
CAGTGCAATGATGAAAGGGCAT335250.12760958265937838No Hit
TCCCTGAGACCCTAACTTGTG332070.1263991472444438No Hit
CTGCAAAGGGAAGCCCTTTCT329100.12526864624370296No Hit
ATCTGGAGGTAAGAAGCACTTT328970.12521916303491634No Hit
AAGTGCTGTCATAGCTGAG328330.12497555339165906No Hit
AACCCGTAGATCCGAACTTGTG326860.12441601249230251No Hit
CAAAGTGCTCATAGTGCAGGTAG324190.12339970351183856No Hit
TACCGGGTGCTGTAGGCTTT323590.12317131947128486No Hit
AGCTCGGTCTGAGGCCCCTCAGT323520.1231446746665536No Hit
TATTGCACTTGTCCCGGCCTGTT314440.11968846285284097No Hit
GGTGCTGTAGGCTTT309560.11783093932300423No Hit
TATTGCACTCGTCCCGGCCTCCT307990.11723333441688871No Hit
AGCAGCATTGTACAGGGCTATGA304770.11600767339925055No Hit
CGTTCGCGCTTTCCCCTG303970.11570316134517895No Hit
AACATTCAACGCTGTCGGTGAGTTT303820.11564606533504053No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC296320.11279126482811931No Hit
TTCAAGTAATCCAGGATAGGC293310.11164553822467493No Hit
CGCGACCTCAGATCAGACGT289640.11024858917662148No Hit
TACAGTACTGTGATAACTGAAG288950.10998594752998474No Hit
TGAGGTAGTAGGTTGTGTGGTTT286410.10901912175830741No Hit
TTCACAGTGGCTAAGTTCTGC286350.10899628335425204No Hit
GCATGGGTGGTTCAGTGGTAGAATTCT286140.10891634894005825No Hit
AAAGTGCTTCCCTTTGGACTG279100.1062366428642282No Hit
ATACCGGGTGCTGTAGGCTTT278610.10605012923110935No Hit
CAAAGCGCTTCTCTTTAGAGTGT274170.104360087331012No Hit
TCAGTGCACTACAGAACTTTGT273380.10405938167761627No Hit
CTGGACGCGAGCCGGGCCCT272210.10361403279853657No Hit
TCGTACCGTGAGTAATAATGCG269600.102620562222128No Hit
TCCCTGAGACCCTAACTTGTGAA268180.1020800533261509No Hit
TTCTCGAGGAAAGAAGCACTTT266620.1014862548207113No Hit
ACCCTGTAGAACCGAATTTGTG265530.10107135714703874No Hit
TGAGATGAAGCACTGTAGCT265160.10093052032203063No Hit
AAGCTGCCAGTTGAAGAACTGT263110.10015020818347217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)