FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004043155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21861993
Sequences flagged as poor quality0
Sequence length13-34
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTGGTTATCTAGCTGTATGA292921613.398668639222416No Hit
TCCCTGAGACCCTAACTTGTGA10318954.720040848974747No Hit
TACCCTGTAGAACCGAATTTGT6598053.018045975954708No Hit
TCTTTGGTTATCTAGCTGTATG6483862.9658137755327245No Hit
TCTTTGGTTATCTAGCTGTAT3785881.731717689233548No Hit
TATTGCACTTGTCCCGGCCTGT1768760.8090570699569797No Hit
TCTTTGGTTATCTAGCTGTATGT1659500.7590799246893913No Hit
AACATTCAACGCTGTCGGTGAGT1616340.739337900254565No Hit
TGAGGTAGTAGGTTGTATGGTT1515950.6934180246055335No Hit
TAGCTTATCAGACTGATGTTGA1365510.6246045362835858No Hit
CAAAGTGCTGTTCGTGCAGGTAG1115070.5100495640996683No Hit
TGAGGTAGTAGGTTGTATAGTT1057070.4835195034597258No Hit
AACCCGTAGATCCGATCTTGT1016150.46480208826340763No Hit
CTTTGGTTATCTAGCTGTATGA998900.4569116822972178No Hit
TACCCTGTAGAACCGAATTTGTG959650.4389581498813946No Hit
CAGTGCAATGTTAAAAGGGCAT955200.43692265384953693No Hit
TAAGGCACGCGGTGAATGCCA953980.43636460774642094No Hit
TCCCTGAGACCCTAACTTGTGAA943220.431442824082873No Hit
TAGCTTATCAGACTGATGTTGAC924950.42308585498129103No Hit
AGAATTGTGGCTGGACATCTGT837360.3830208892665916No Hit
TTCAAGTAATCCAGGATAGGCT799060.36550190094745705No Hit
CATTGCACTTGTCTCGGTCTGA785790.3594320060389737No Hit
TACCCTGTAGATCCGAATTTGT746300.3413686940618817No Hit
AACCCGTAGATCCGATCTTGTG736700.3369775116111326No Hit
TATTGCACTCGTCCCGGCCTCC724850.3315571457734892No Hit
TGAGGTAGTAGATTGTATAGTT720900.32975035716094137No Hit
AACCCGTAGATCCGAACTTGTG681850.3118883077128421No Hit
GGGTGCTGTAGGCTTT667360.3052603667012427No Hit
GGTGCTGTAGGCTTT652370.29840371827033335No Hit
ATCGTGCATCCCTTTAGAGTGT640830.293125151032662No Hit
CGGGTGCTGTAGGCTTT635490.29068255579443286No Hit
TATGGCTTTTTATTCCTATGTGA583510.26690613248298084No Hit
CCGGGTGCTGTAGGCTTT556280.25445072642736644No Hit
AACCCGTAGATCCGATCTTGTGT540280.24713208900945124No Hit
TCCCTGAGACCCTAACTTGTG518460.23715129723076941No Hit
TGTGCAAATCCATGCAAAACTGA487070.22279304544649703No Hit
CAAGTGCTTCCTTTTAGAGGGT484680.22169982398219595No Hit
TGAGGTAGTAGTTTGTGCTGTT484270.22151228389836186No Hit
GGGTGCTGTAGGCTT462920.21174647709383132No Hit
TAAAGTGCTGACAGTGCAGATA447970.2049081252564668No Hit
CGGGTGCTGTAGGCTT440430.20145921737327427No Hit
TTCACAGTGGCTAAGTTCTGC432100.19764895176757213No Hit
ACCGGGTGCTGTAGGCTTT430070.19672039964517418No Hit
AAGTGCTGTCATAGCTGAGGTC420520.19235208793635603No Hit
TTTGGCAATGGTAGAACTCACACT402710.18420552966053919No Hit
TACAGTACTGTGATAACTGAAG394220.18032207768065792No Hit
TAAGGCACGCGGTGAATGCCAA380670.17412410661736102No Hit
TTGTGCTTGATCTAACCATGT379750.1737032849658309No Hit
CTCTTGAGGGAAGCACTTTCTGT375600.17180501338555915No Hit
ACCCTGTAGAACCGAATTTGTGT367460.16808165659919477No Hit
AACATTCAACGCTGTCGGTGAGTTT360280.16479741805790535No Hit
AACATTCAACGCTGTCGGTGAGTT358600.16402896112902424No Hit
TGTAAACATCCTTGACTGGAAGCT355630.16267043905832373No Hit
CACCCGTAGAACCGACCTTGCG350780.1604519770910182No Hit
AGCAGCATTGTACAGGGCTATGA347260.15884187685907683No Hit
TCAGTGCACTACAGAACTTTGT345130.15786758325281688No Hit
CCGGGTGCTGTAGGCTT336160.15376457215039818No Hit
TATACAAGGGCAAGCTCTCTGT327110.14962496786088988No Hit
TACTGCATCAGGAACTGATTGGA320200.14646423132602776No Hit
AAGTGCTGTCATAGCTGAGGTCA318750.1458009798100292No Hit
TGAGGTAGTAGTTTGTACAGTT316940.14497305895212756No Hit
TCTTTGGTTATCTAGCTGTATGAA315080.1441222673522949No Hit
TACCCTGTAGAACCGAATTTGTGT312730.14304734248153864No Hit
TTAAGGCACGCGGTGAATGCCA308150.1409523825206604No Hit
AAGTGCTGTCATAGCTGAGGTCT304400.13923707687583653No Hit
TACCCTGTAGAACCGAATTTGTGA302470.13835426623730052No Hit
ACCCTGTAGAACCGAATTTGTG296690.13571040847007865No Hit
TCCCTGAGACCCTAACTTGTGAAA293760.13437018299292292No Hit
TGTCTGAGCGTCGCTT289560.13244904067072016No Hit
TCAGTGCATCACAGAACTTTGT288430.13193216190307994No Hit
CAAAGTGCTTACAGTGCAGGTAG279390.12779713176195784No Hit
TCCCTGAGACCCTTTAACCTGT275160.12586226699459652No Hit
AACCCGTAGATCCGAACTTGT273800.12524018281407373No Hit
TGTAAACATCCTACACTCTCAGCT267600.12240421081463157No Hit
TTTGGCAATGGTAGAACTCACA266840.12205657553728061No Hit
TGTAAACATCCCCGACTGGAAGCT264370.12092676088588995No Hit
ATAAAGCTAGATAACCGAAAGT260610.11920688109267989No Hit
TCTTTGGTTATCTAGCTGTATGAT259640.11876318869921879No Hit
TACCGGGTGCTGTAGGCTTT255050.11666365458995437No Hit
AACCCGTAGATCCGATCTTGTGA250990.11480655034515838No Hit
CTGTCTGAGCGTCGCTT240590.1100494360235135No Hit
AACCCGTAGATCCGAACTTGTGA238270.1089882335979158No Hit
ATCCCGGACGAGCCCCCA235860.10788586383684233No Hit
TCCCTGAGACCCTAACTTGT233450.10678349407576884No Hit
CGTACGCGGGTTTAAACGA233030.10659137984354856No Hit
ACCGGGTGCTGTAGGCTT231030.10567655016630918No Hit
CCTGTCTGAGCGTCGCTT230440.10540667541152356No Hit
TAAAGTGCTGACAGTGCAGAT228780.10464736677941486No Hit
TAAAGTGCTTATAGTGCAGGTAG226830.10375540784410645No Hit
TCCCTGAGACCCTTTAACCTGTG226590.10364562828283772No Hit
TCGGATCCGTCTGAGCTTGGCT226550.1036273316892929No Hit
TATGGCACTGGTAGAATTCACT226510.10360903509574813No Hit
ATCCCACTTCTGACACCA220050.10065413523826489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)