FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004043156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12600226
Sequences flagged as poor quality0
Sequence length13-34
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAGACCCTAACTTGTGA3912733.105285571861965No Hit
TCTTTGGTTATCTAGCTGTATGA2138781.697413998764784No Hit
CATTGCACTTGTCTCGGTCTGA1857971.474552916749271No Hit
CAAAGTGCTGTTCGTGCAGGTAG1773551.4075541184737481No Hit
AACATTCAACGCTGTCGGTGAGT1613901.280850041896074No Hit
TAGCTTATCAGACTGATGTTGAC1175060.9325705745277902No Hit
TATTGCACTTGTCCCGGCCTGT1120820.8895237275902829No Hit
AACCCGTAGATCCGATCTTGTG1048590.8321993589638789No Hit
TAGCTTATCAGACTGATGTTGA1031500.8186361101777064No Hit
AACCCGTAGATCCGATCTTGT930160.7382089813309698No Hit
AACCCGTAGATCCGATCTTGTGT800210.6350759105431919No Hit
AAGTGCTGTCATAGCTGAGGTC727490.5773626600030825No Hit
TACCCTGTAGAACCGAATTTGT690760.5482123892063523No Hit
AGAATTGTGGCTGGACATCTGT665080.5278318023819573No Hit
CAGTGCAATGTTAAAAGGGCAT643400.5106257617918917No Hit
TCAGTGCACTACAGAACTTTGT591160.46916618797154913No Hit
TCTTTGGTTATCTAGCTGTATG585530.4646980141467304No Hit
GGGTGCTGTAGGCTT564060.44765863723396704No Hit
ATCGTGCATCCCTTTAGAGTGT552060.43813499853097876No Hit
AAGTGCTGTCATAGCTGAGGTCT531610.4219051309079695No Hit
CGGGTGCTGTAGGCTT498750.39582623359295305No Hit
TCCCTGAGACCCTAACTTGTGAA478510.379763029647246No Hit
CCGGGTGCTGTAGGCTT457320.36294587097088576No Hit
GGGTGCTGTAGGCTTT453080.3595808519624965No Hit
TTCTCCAAAAGAAAGCACTTTCT450650.3576523151251414No Hit
CAAGTGCTTCCTTTTAGAGGGT448000.3555491782448982No Hit
ACCGGGTGCTGTAGGCTT446040.3539936505900767No Hit
TGAGGTAGTAGGTTGTATAGTT441080.3500572132595082No Hit
AACATTCAACGCTGTCGGTGAGTT437740.3474064671538431No Hit
TCTTTGGTTATCTAGCTGTAT431870.34274782055496467No Hit
GGTGCTGTAGGCTTT418970.33250990894925214No Hit
TAAAGTGCTGACAGTGCAGATA413280.3279941169309185No Hit
TGAGGTAGTAGGTTGTATGGTT412370.3272719076626086No Hit
TGTAAACATCCTTGACTGGAAGCT402400.3193593511735424No Hit
AAATCTCTGCAGGCAAATGTGA390890.3102245943842594No Hit
AACCCGTAGATCCGATCTTGTGA384850.305431029570422No Hit
TCTTTGGTTATCTAGCTGTATGT377600.29967716452069987No Hit
TGTGCAAATCCATGCAAAACTGA375320.2978676731671321No Hit
CGGGTGCTGTAGGCTTT372520.2956454908031015No Hit
AAGTGCTGTCATAGCTGAGGTCA371270.2946534451048735No Hit
TGAGGTAGTAGATTGTATAGTT343610.27270145789448536No Hit
TACTGCATCAGGAACTGATTGGA337000.26745552024225594No Hit
AACATTCAACGCTGTCGGTGAGTTT336600.26713806561882303No Hit
ATCTGGAGGTAAGAAGCACTTTCT335890.2665745836622295No Hit
CAAAGTGCTTACAGTGCAGGTAG320210.2541303624236581No Hit
CCGGGTGCTGTAGGCTTT316030.25081296160878386No Hit
TAAAGTGCTGACAGTGCAGAT300140.23820207669291013No Hit
TCCCTGAGACCCTAACTTGTG298940.2372497128226113No Hit
TCAGTGCATCACAGAACTTTGT285200.22634514650768964No Hit
CGTACGCGGGTTTAAACGA275440.21859925369592578No Hit
ACCGGGTGCTGTAGGCTTT269520.21390092526911822No Hit
TTCAAGTAATCCAGGATAGGCT264130.20962322421835927No Hit
TGTGCAAATCCATGCAAAACTG263630.20922640593906808No Hit
TGAGATGAAGCACTGTAGCTCT256750.20376618641602143No Hit
TGGAAGACTAGTGATTTTGTTGTT253850.2014646403961326No Hit
AAGTGCTGTCATAGCTGAGGT249050.19765518491493722No Hit
AACATTCAACGCTGTCGGTGAG243450.19321082018687602No Hit
AACCCGTAGATCCGAACTTGTG243120.19294892012254383No Hit
TAAGGCACGCGGTGAATGCCA243120.19294892012254383No Hit
TGTAAACATCCCCGACTGGAAGCT218460.1733778425879028No Hit
TGTAAACATCCTACACTCTCAGCT218130.17311594252357063No Hit
CTCTTGAGGGAAGCACTTTCTGT217060.17226675140588749No Hit
TATTGCACTCGTCCCGGCCTCC205410.16302088549840296No Hit
TGTAAACATCCTCGACTGGAAGCT201250.15971935741470034No Hit
GGTGCTGTAGGCTT199090.15800510244816246No Hit
ATCCCACTTCTGACACCA197330.15660830210505747No Hit
CAAGTGCTTCCTTTTAGAGGGTT197000.1563464020407253No Hit
TGTAAACATCCTTGACTGGAAGC196580.15601307468612072No Hit
CAAAGTGCTGTTCGTGCAGGTAGT195860.15544165636394142No Hit
TAAAGTGCTTATAGTGCAGGTAG191840.15225123739844032No Hit
AGCTCGGTCTGAGGCCCCTCAGT191100.15166394634508937No Hit
TACCGGGTGCTGTAGGCTT186950.14837035462697257No Hit
TGTCTGAGCGTCGCTT184240.14621959955321437No Hit
AACATTCATTGCTGTCGGTGGGTT179890.14276728052338108No Hit
CAACGGAATCCCAAAAGCAGCT178250.141465716567306No Hit
AAGCTGCCAGTTGAAGAACTGT170690.13546582418442335No Hit
AAAGTGCTTCCCTTTGGACTGT168610.13381506014257205No Hit
AGCAGCATTGTACAGGGCTATGA168180.13347379642238164No Hit
AACCCGTAGATCCGAACTTGT167840.13320395999246365No Hit
CTGTCTGAGCGTCGCT167130.13264047803587015No Hit
GTACAGTACTGTGATAACTGA166400.13206112334810502No Hit
TACAGTACTGTGATAACTGAAG164820.1308071775855449No Hit
CAGTGCAATGATGAAAGGGCAT163420.1296960864035296No Hit
TCCCTGAGACCCTAACTTGT162750.1291643499092794No Hit
CAAAGTGCCTCCCTTTAGAGTGT160910.12770405864148787No Hit
CCTGTCTGAGCGTCGCT160830.12764056771680127No Hit
TGTCTGAGCGTCGCT158900.1261088491587373No Hit
TAGCTTATCAGACTGATGTTG158150.12551362173980055No Hit
ACGCGACCTCAGATCAGACGT157100.12468030335328906No Hit
TGAGATGAAGCACTGTAGCTC154570.12267240286007568No Hit
TGTAAACATCCCCGACTGGAAGC151180.1199819749264815No Hit
ATACCGGGTGCTGTAGGCTT151060.11988673853945159No Hit
CAAAGTGCTGTTCGTGCAGGTA149890.11895818376591023No Hit
TGTAAACATCCTACACTCTCAGC148870.1181486744761562No Hit
ATCCCGGACGAGCCCCCA147360.11695028327269685No Hit
TCGTACCGTGAGTAATAATGCG146000.11587093755302483No Hit
CTGTCTGAGCGTCGCTT145930.11581538299392406No Hit
GGCCGGTTAGCTCAGTTGGT145080.11514079191912907No Hit
TCCCCGGCACCTCCACCA144450.11464080088722217No Hit
AACATTCATTGCTGTCGGTGGGT143230.1136725642857517No Hit
TTCACAGTGGCTAAGTTCTGC140230.1112916546100046No Hit
TAAGGCACGCGGTGAATGCCAA139640.11082340904044101No Hit
CACCCGTAGAACCGACCTTGCG139320.11056944534169467No Hit
CAACGGAATCCCAAAAGCAGCTG137990.10951390871878013No Hit
TGAGGTAGTAGGTTGTATAGT137590.10919645409534719No Hit
GGGGATGTAGCTCAGTGGT136500.1083313902464924No Hit
AGTGGGTTATCAGAAC136480.10831551751532076No Hit
AAATCTCTGCAGGCAAATGTG136360.10822028112829087No Hit
CAAAGCGCTCCCCTTTAGAGGT135930.10787901740810046No Hit
GCCTGTCTGAGCGTCGCT134670.10687903534428668No Hit
CGACTCTTAGCGGTGGATCACT133890.10625999882859244No Hit
TACCCTGTAGATCCGAATTTGT133450.1059107987428162No Hit
TCCCTGAGACCCTTTAACCTGT133240.10574413506551392No Hit
AACATTCAACGCTGTCGGTGA132810.10540287134532349No Hit
TATTGCACTTGTCCCGGCCTGTA131520.10437908018475224No Hit
CTCGACACAAGGGTTTGT130810.10381559822815876No Hit
CCTGTCTGAGCGTCGCTT130660.10369655274437141No Hit
TGAGGTAGTAGTTTGTACAGTT129230.10256165246559865No Hit
CGACTCTTAGCGGTGGATCACTC126630.1004981974132845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)