FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004043157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14079091
Sequences flagged as poor quality0
Sequence length13-34
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGCACTTGTCCCGGCCTGT2996032.1279995988377376No Hit
CAAAGTGCTGTTCGTGCAGGTAG1833281.302129519583331No Hit
CAGTGCAATGTTAAAAGGGCAT1688831.1995305662844284No Hit
TCCCTGAGACCCTAACTTGTGA1341300.9526893462085015No Hit
CATTGCACTTGTCTCGGTCTGA1148970.8160825155544489No Hit
TGTGCAAATCCATGCAAAACTGA1082980.7692115918563208No Hit
TAGCTTATCAGACTGATGTTGAC1048900.7450054836636825No Hit
GGGTGCTGTAGGCTTT928040.6591618734476536No Hit
AACATTCAACGCTGTCGGTGAGT885790.6291528338015573No Hit
TCTTTGGTTATCTAGCTGTATGA857540.6090876179435164No Hit
TAGCTTATCAGACTGATGTTGA797340.5663291756548772No Hit
CAAAGTGCTTACAGTGCAGGTAG792600.5629624810294926No Hit
CGGGTGCTGTAGGCTTT790420.5614140856110668No Hit
CGGGTGCTGTAGGCTT742730.527541160150183No Hit
TAAAGTGCTGACAGTGCAGATA653690.4642984408581492No Hit
CCGGGTGCTGTAGGCTTT653550.46419900262026864No Hit
TATTGCACTCGTCCCGGCCTCC639090.4539284531934626No Hit
CCGGGTGCTGTAGGCTT630610.4479053370704117No Hit
AACCCGTAGATCCGATCTTGTG581450.41298831011178205No Hit
TGTAAACATCCTCGACTGGAAGCT568980.4041312042091354No Hit
TTCAAGTAATCCAGGATAGGCT550040.3906786311701515No Hit
GGGTGCTGTAGGCTT540340.3837889818312844No Hit
TAAAGTGCTTATAGTGCAGGTAG505730.35920642888095544No Hit
TGTAAACATCCTTGACTGGAAGCT496110.3523736013923058No Hit
GGTGCTGTAGGCTTT491790.3493052214805629No Hit
TGAGGTAGTAGGTTGTATAGTT467900.3323367964593737No Hit
AGAATTGTGGCTGGACATCTGT463970.32954542306744095No Hit
AACATTCAACGCTGTCGGTGAGTT455390.32345127963161824No Hit
ACCGGGTGCTGTAGGCTT454200.32260605460963354No Hit
TTCACAGTGGCTAAGTTCTGC449700.3194098255349013No Hit
ACCGGGTGCTGTAGGCTTT448060.3182449776054434No Hit
TGTAAACATCCTACACTCTCAGCT419650.29806611804696764No Hit
TAAAGTGCTGACAGTGCAGAT407860.2896919978711694No Hit
CAAAGTGCTCATAGTGCAGGTAG381280.27081293813641805No Hit
TATTGCACTTGTCCCGGCCTGTA380910.2705501370791623No Hit
TGTAAACATCCTCGACTGGAAGC367810.26124555910605307No Hit
ATCCCACTTCTGACACCA365840.2598463210444481No Hit
TGTCTGAGCGTCGCTT359820.25557047681558415No Hit
CTGTCTGAGCGTCGCTT343860.24423451769720075No Hit
TGTGCAAATCTATGCAAAACTGA335170.23806224421732908No Hit
TACCGGGTGCTGTAGGCTTT330340.2346316250104499No Hit
ATCTGGAGGTAAGAAGCACTTTCT326370.2318118406934084No Hit
TACCGGGTGCTGTAGGCTT320470.22762122923987066No Hit
TCAGTGCACTACAGAACTTTGT309550.21986504668518725No Hit
CGACTCTTAGCGGTGGATCACT303140.21531219593651324No Hit
ATCCCGGACGAGCCCCCA290700.2064763982276981No Hit
AACATTCAACGCTGTCGGTGAGTTT290700.2064763982276981No Hit
ATACCGGGTGCTGTAGGCTTT287540.20423193514410837No Hit
CGACTCTTAGCGGTGGATCACTC285360.2026835397256826No Hit
TTTGGCAATGGTAGAACTCACACT284680.20220055399883416No Hit
AGCAGCATTGTACAGGGCTATGA284100.20178859558475756No Hit
ATACCGGGTGCTGTAGGCTT274480.19495576809610793No Hit
TATTGCACTTGTCCCGGCCTGTT273940.19457222060714005No Hit
CCTGTCTGAGCGTCGCTT273260.19408923488029162No Hit
CTGTCTGAGCGTCGCT273120.1939897966424111No Hit
CCTGTCTGAGCGTCGCT271940.19315167435170352No Hit
CAGTGCAATGATGAAAGGGCAT269020.1910776768187662No Hit
AACCCGTAGATCCGAACTTGTG265980.1889184465105027No Hit
TACAGTACTGTGATAACTGAAG262380.18636146325071698No Hit
ATCCCACTCCTGACACCA262060.18613417584984712No Hit
CGACTCTTAGCGGTGGATC258410.18354167893367546No Hit
AACATTCATTGCTGTCGGTGGGTT258330.18348485708345802No Hit
ACGCGACCTCAGATCAGACGT248060.17619035206179148No Hit
AGCTACATTGTCTGCTGGGTTTC244520.17367598518966884No Hit
AGCTCGGTCTGAGGCCCCTCAGT243100.17266739734830894No Hit
AATTGCACGGTATCCATCTGTA238430.16935042184186463No Hit
CTCGACACAAGGGTTTGT234730.1667224112693071No Hit
TGTAAACATCCCCGACTGGAAGCT233930.16615419276713247No Hit
TGTAAACATCCTACACTCTCAGC228990.16264544351620427No Hit
CGACTCTTAGCGGTGGA221440.15728288140193142No Hit
TCCGGCTCGAAGGACCA219110.15562794501434787No Hit
TGAGGTAGTAGATTGTATAGTT217600.1545554325914933No Hit
CGCCTGTCTGAGCGTCGCT210180.1492852059838238No Hit
TGTAAACATCCTTGACTGGAAGC207050.14706205109406567No Hit
AACCCGTAGATCCGATCTTGTGA203670.14466132792237796No Hit
AACCCGTAGATCCGATCTTGT201590.14318395981672397No Hit
TCAGTGCATCACAGAACTTTGT199300.14155743435424914No Hit
AAGTGCTGTCATAGCTGAGGTC195800.13907147840723524No Hit
GCCTGTCTGAGCGTCGCT189050.134277134795137No Hit
TGATTGTCCAAACGCAATTCTTG184700.13118744668956256No Hit
ACGCGACCTCAGATCAGA184480.13103118660146454No Hit
TTCAAGTAATTCAGGATAGGTT182830.12985923594072943No Hit
TGTGCAAATCCATGCAAAACTG178260.12661328774705696No Hit
ACGCGACCTCAGATCAGAC177120.12580357638145814No Hit
TGTAAACATCCCCGACTGGAAGC173010.12288435382653609No Hit
ACGCGACCTCAGATCAGACG172630.12261445003800317No Hit
CGCCTGTCTGAGCGTCGCTT169530.12041260334207655No Hit
TCCCCGGCACCTCCACCA161090.11441789814413444No Hit
TATGGCACTGGTAGAATTCACT160290.11384967964195984No Hit
TCGACACAAGGGTTTGT159740.1134590294217148No Hit
GCCTGTCTGAGCGTCGCTT159550.11332407752744833No Hit
CGACACAAGGGTTTGT158440.11253567435568106No Hit
TTTGGCACTAGCACATTTTTGCT157710.11201717497244673No Hit
TCTTTGGTTATCTAGCTGTATG156740.11132821003856001No Hit
ACGCGACCTCAGATCAGACGTGGC155890.1107244778799995No Hit
CAGTGCAATGTTAAAAGGGCATT154860.1099928965584497No Hit
CAACGGAATCCCAAAAGCAGCTG154690.1098721501267376No Hit
AACCCGTAGATCCGATCTTGTGT153870.1092897261620086No Hit
TCTTTGGTTATCTAGCTGTAT149350.10607929162472207No Hit
TAGCAGCACGTAAATATTGGCG149290.10603667523705898No Hit
TCGAGGAGCTCACAGTCTAGT148230.10528378572167764No Hit
TATTGCACTTGTCCCGGCCTGTAA146500.10405501321072504No Hit
TCGTACGACTCTTAGCGGTGGATC144810.1028546516248812No Hit
ACGCGACCTCAGATCAG143310.1017892419333038No Hit
AACATTCATTGCTGTCGGTGGGT142670.10133466713156411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)