FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004043159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21720535
Sequences flagged as poor quality0
Sequence length13-34
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGCGGGTTTAAACGA219759710.117600694458034No Hit
CGTACGCGGAATAGTTTAAACTGT10302124.743032342435396No Hit
AGAATTGTGGCTGGACATCTGT2634181.212760182932879No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1487820.6849831277176184No Hit
GTTCTACAGTCCGACGATC1273140.586145783241527Illumina DpnII expression Sequencing Primer (100% over 19bp)
TATTGCACTTGTCCCGGCCTGT1226290.5645763329494415No Hit
ACCGGGTGCTGTAGGCTT1178610.542624755789855No Hit
ACCGGGTGCTGTAGGCTTT828020.3812152877449842No Hit
CCTGTCTGAGCGTCGCT743210.34216928818742265No Hit
CGTACGCGGGTTTAACGA727280.33483521469429733No Hit
CCGGGTGCTGTAGGCTT646440.29761697858731384No Hit
CAGAGTTCTACAGTCCGACGATC617730.2843990721223027Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGTACGCGGGTTAAACGA586420.26998414173499874No Hit
CGTACGCGGGTTTAAACG585140.2693948376501776No Hit
CCGGGTGCTGTAGGCTTT557610.2567201958883609No Hit
CGCCTGTCTGAGCGTCGCT552630.2544274346833538No Hit
GCCTGTCTGAGCGTCGCT545100.25096066924686705No Hit
TCAGAGTTCTACAGTCCGACGATC531510.2447039172838054Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCTCGGTCTGAGGCCCCTCAGT508480.234101047695188No Hit
AGTTCTACAGTCCGACGATC498930.22970428674984295No Hit
CGTACGCGGAATAGTTAAACTGT485370.22346134660126926No Hit
CATTGCACTTGTCTCGGTCTGA484070.22286283464012283No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG477080.21964468186442002No Hit
CGTACGCGGAATAGTTTAACTGT472650.21760513725835942No Hit
CGTACGCGGGTTTAAA457740.21074066545782597No Hit
CGGGTGCTGTAGGCTTT400400.18434168403310508No Hit
CGGGTGCTGTAGGCTT398610.18351757910198804No Hit
TATTGCACTCGTCCCGGCCTCC395720.18218704097297786No Hit
ACCACTGACCGTTGACTGTACC382720.17620192136151344No Hit
CGTACGCGGGTTTAAAC376030.17312188673069057No Hit
CTGTCTGAGCGTCGCT365220.16814502957684974No Hit
CTACAGTCCGACGATC353540.16276762980285706Illumina DpnII expression Sequencing Primer (100% over 16bp)
TACCGGGTGCTGTAGGCTT352720.16239010687351854No Hit
ATACCGGGTGCTGTAGGCTT340650.15683315351118193No Hit
CGTACGCGGGCTTAAACGA334360.15393727640686566No Hit
GGGAATACCGGGTGCTGTAGGCTT328770.15136367497393596No Hit
TCTTGGTCGTACGCGGAATAGTTTAAACTGT316860.145880384622202No Hit
CTTGGTCGTACGCGGAATAGTTTAAACTGT316220.14558573257979143No Hit
TGGGAATACCGGGTGCTGTAGGCTT312770.143997373913672No Hit
TACCGGGTGCTGTAGGCTTT303710.13982620593829756No Hit
GTACGCGGAATAGTTTAAACTGT302600.13931516880224176No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC296500.13650676652301613No Hit
TAGAGTTCTACAGTCCGACGATC291030.13398841234803838Illumina DpnII expression Sequencing Primer (95% over 23bp)
GGAATACCGGGTGCTGTAGGCTT289730.13338990038689194No Hit
ATACCGGGTGCTGTAGGCTTT285710.13153911724550063No Hit
GAATACCGGGTGCTGTAGGCTT285350.1313733754716447No Hit
CTCATCTTGGTCGTACGCGGAATAGTTTAAA284510.13098664466598084No Hit
TCGGATCCGTCTGAGCTTGGCT283680.13060451779847965No Hit
GGGAATACCGGGTGCTGTAGGCTTT281240.12948115688678938No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT281100.12941670175251208No Hit
CCCCCCACTGCTAAATTTGACTGGCTT279180.12853274562528041No Hit
GAATACCGGGTGCTGTAGGCTTT275780.1269674066499743No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC272280.12535602829304157No Hit
GGAATACCGGGTGCTGTAGGCTTT271990.1252225140863243No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA269330.12399786653505543No Hit
GGGTGCTGTAGGCTTT267930.12335331519228232No Hit
CGACTCTTAGCGGTGGATCACTC263860.12147951236007769No Hit
GTACGCGGGTTTAAACGA259620.11952744257910775No Hit
CGTACGCGGGTTTAAAGA252810.11639216069033291No Hit
TGGGAATACCGGGTGCTGTAGGCTTT248060.11420529006306705No Hit
TTCTACAGTCCGACGATC247950.11415464674327774Illumina DpnII expression Sequencing Primer (100% over 18bp)
CGACTCTTAGCGGTGGATCACTCGGC237560.10937115499226883No Hit
CGTACGCGGATTTAAACGA233690.1075894309233175No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC232660.107115225292563No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT230530.10613458646391537No Hit
TCTACAGTCCGACGATC226230.10415489305396944Illumina DpnII expression Sequencing Primer (100% over 17bp)
CGCGGGACATGTGGCGTACGGA222480.10242841624297007No Hit
TCAGTGCATCACAGAACTTTGT221410.10193579485956493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)