FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004043160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62591891
Sequences flagged as poor quality0
Sequence length13-34
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCAAATCCATGCAAAACTG23046413.682012099618463No Hit
CGTACGCGGGTTTAAACGA15124902.4164312274891966No Hit
TAGCTTATCAGACTGATGTTGA11781561.8822821633556333No Hit
CGTACGCGGAATAGTTTAAACTGT11269441.8004632580920106No Hit
TAGCTTATCAGACTGATGTTGAC7849021.253999499711552No Hit
TTCTCCAAAAGAAAGCACTTTCTG7442981.1891284767223282No Hit
TGTGCAAATCCATGCAAAACTGA5405060.863539975170266No Hit
ACCGGGTGCTGTAGGCTT5268400.8417064760034171No Hit
TATTGCACTTGTCCCGGCCTGT5019880.8020016522587566No Hit
TGTGCAAATCTATGCAAAACTG4073860.6508606681974187No Hit
CAAGTGCTTCCTTTTAGAGGGT3994830.6382344319969498No Hit
AAGTGCTGTCATAGCTGAGGTC3944850.6302493720792044No Hit
AACATTCAACGCTGTCGGTGAGT3941540.6297205495836513No Hit
CAAAGTGCTGTTCGTGCAGGTAG3679170.587802979143097No Hit
ATCTGGAGGTAAGAAGCACTTTCT3106830.49636301929270676No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2998830.4791083880178664No Hit
CAGTGCAATGTTAAAAGGGCAT2957410.4724909173937563No Hit
CATTGCACTTGTCTCGGTCTGA2955140.47212825060677593No Hit
AAGTGCTGTCATAGCTGAGGTCT2830290.45218157732285164No Hit
GTTCTACAGTCCGACGATC2724760.435321565855871Illumina DpnII expression Sequencing Primer (100% over 19bp)
AAACCGTTACCATTACTGAGT2686540.4292153435658303No Hit
AAACCGTTACCATTACTGAGTT2623970.4192188409837306No Hit
ATCGTGCATCCCTTTAGAGTGT2531040.40437186983214807No Hit
ACCGGGTGCTGTAGGCTTT2407430.3846233052776757No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC2261590.3613231624524653No Hit
CAAAGTGCTTACAGTGCAGGTAG2022890.3231872320329801No Hit
TTCTCCAAAAGAAAGCACTTTCT1962340.31351345496176175No Hit
AAGTGCTGTCATAGCTGAGGT1953060.3120308347929607No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG1908390.30489412757956136No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC1822480.29116870746084345No Hit
TACAGTATAGATGATGTACT1800260.2876187268411495No Hit
TACAGTACTGTGATAACTGAAG1797480.2871745798509267No Hit
CCGGGTGCTGTAGGCTT1702100.2719361841935723No Hit
TAAAGTGCTTATAGTGCAGGTAG1701870.27189943821956103No Hit
CGGGTGCTGTAGGCTT1610550.25730968888605715No Hit
TGATTGTCCAAACGCAATTCTTG1579170.25229625991008964No Hit
TTCAAGTAATCCAGGATAGGCT1562160.2495786554843023No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC1535530.24532411075421898No Hit
AAGTGCTGTCATAGCTGAGG1472130.23519500313547007No Hit
TCAAAACTGAGGGGCATTTTCT1419510.22678816334211727No Hit
GGGTGCTGTAGGCTTT1398960.2235049904467657No Hit
CGGGTGCTGTAGGCTTT1377860.22013394674399597No Hit
AAGTGCTGTCATAGCTGAGGTCA1329940.21247800294130753No Hit
TGAGGTAGTAGGTTGTATAGTT1328390.21223036702949272No Hit
CTACAAAGGAAAGCGCTTTCTG1294990.2068942125426439No Hit
TAGCAGCACGTAAATATTGGCG1231690.19678108143433465No Hit
CCGGGTGCTGTAGGCTTT1231510.1967523237155433No Hit
AGTGGGTTATCAGAAC1222260.19527449649987408No Hit
AACATTCAACGCTGTCGGTGAGTTT1209820.1932870186011795No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC1201470.1919529799794673No Hit
TCCCTGAGACCCTAACTTGTGA1196820.1912100722440228No Hit
TATTGCACTCGTCCCGGCCTCC1179580.1884557218442242No Hit
TCAGTGCACTACAGAACTTTGT1170360.1869826875816869No Hit
TAAAGTGCTGACAGTGCAGATA1164210.18600013218964737No Hit
TCTTTGGTTATCTAGCTGTATGA1145470.18300613413325378No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCG1135260.18137493241736377No Hit
TGTGCAAATCTATGCAAAACTGA1110310.17738879306266686No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1103510.1763023903527695No Hit
TGAGGTAGTAGATTGTATAGTT1070500.1710285442566354No Hit
AGTTCTACAGTCCGACGATC1052630.16817354184106692No Hit
CAAGTGCTTCCTTTTAGAGGGTT1051760.1680345462002418No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTC1047240.16731240792836888No Hit
TCGGATCCGTCTGAGCTTGGCT1029620.16449734678889955No Hit
ATCTGGAGGTAAGAAGCACTTTC1018600.16273673533844824No Hit
CAAAGTGCTCATAGTGCAGGTAG979640.15651228687115398No Hit
GGGTGCTGTAGGCTT962930.1538426119766856No Hit
CTCTTGAGGGAAGCACTTTCTGT956580.15282810356376675No Hit
GAGTGCCTTCTTTTGGAGCGTT944740.15093648472771018No Hit
CTACAAAGGGAAGCACTTTCTC931930.14888989374038883No Hit
CTACAAAGGGAAGCCCTTTCT931650.14884515951115776No Hit
TTCTCGAGGAAAGAAGC925490.14786100646807426No Hit
ATCTGGAGGTAAGAAGCACTTT924840.14775715915021642No Hit
AGCTACATTGTCTGCTGGGTTT910270.14542938157915694No Hit
TGTAAACATCCTTGACTGGAAGCT908310.14511624197453948No Hit
AGCTACATTGTCTGCTGGGTTTC903240.14430623289524838No Hit
AACATTCAACGCTGTCGGTGAG889190.14206153317847514No Hit
AAACCGTTACCATTACTGAGTTT870820.1391266482107083No Hit
ACCGGGTGCTGTAGGCT870600.13909149988774105No Hit
TTCTCGAGGAAAGAAGCACTTT863200.1379092381152057No Hit
AAGTGCTGTCATAGCTGAG852970.13627484109722776No Hit
TAGCTTATCAGACTGATGTTG848170.13550796859612374No Hit
CAAAGTGCCTCCCTTTAGAGTGT847700.1354328789970573No Hit
CAGTGCAATGATGAAAGGGCAT823250.1315266221945587No Hit
TAAAGTGCTGACAGTGCAGAT790360.12627194791095223No Hit
TTCAAGTAATTCAGGATAGGTT785680.12552424722237582No Hit
CAAAGCGCTTCTCTTTAGAGTGT778380.12435796196028012No Hit
CTCCAGAGGGAAGTACTTTCT760560.12151094779993146No Hit
CTACAGTCCGACGATC752390.12020566689701068Illumina DpnII expression Sequencing Primer (100% over 16bp)
CAAAGCGCTCCCCTTTAGAGGT739850.11820221248787642No Hit
CTCTAGAGGGAAGCGCTTTCTG736780.11771173361737863No Hit
CAGAGTTCTACAGTCCGACGATC716050.11439980300323568Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCG715150.11425601440927867No Hit
ATACCGGGTGCTGTAGGCTT707470.11302901840751226No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCC705970.11278937075091724No Hit
TACCGGGTGCTGTAGGCTT704960.1126280079954766No Hit
CAACGGAATCCCAAAAGCAGCTG681630.10890068810990229No Hit
AGCAGCATTGTACAGGGCTATGA674960.10783505486357649No Hit
TATTGCACTTGTCCCGGCCTGTA669710.10699628806549398No Hit
ATCCCACTTCTGACACCA654180.10451513599421369No Hit
ATCACACAAAGGCAACTTTTGT644380.10294943797112632No Hit
TTCTCGAGGAAAGAAGCACTTTC632610.10106900269237751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)