FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004043161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36308124
Sequences flagged as poor quality0
Sequence length13-34
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGCACTTGTCCCGGCCTGT15122434.165026537862436No Hit
CGTACGCGGGTTTAAACGA13918443.8334230653172825No Hit
CAAAGTGCTTACAGTGCAGGTAG10286332.8330656797360283No Hit
TGTGCAAATCCATGCAAAACTGA7779842.1427270657112443No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC7468582.0569996951646417No Hit
TAGCTTATCAGACTGATGTTGA6425991.7698490839130105No Hit
TAGCTTATCAGACTGATGTTGAC5894811.623551247098308No Hit
TAAAGTGCTTATAGTGCAGGTAG4914371.3535180170696783No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC4791541.3196881226912194No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG4132001.138037316386823No Hit
CGTACGCGGAATAGTTTAAACTGT3150180.8676240061315202No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC3039520.8371459786795925No Hit
TGTGCAAATCCATGCAAAACTG2973840.8190563632535792No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG2857580.7870359812586295No Hit
TATTGCACTTGTCCCGGCCTGTAA2135250.5880915246406011No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT2120410.5840042851015932No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA1850960.5097922437413731No Hit
TGAGGTAGTAGGTTGTATAGTT1765320.48620523605130356No Hit
TGAGGTAGTAGATTGTATAGTT1626850.4480677657705477No Hit
GTTCTACAGTCCGACGATC1494010.4114809126464369Illumina DpnII expression Sequencing Primer (100% over 19bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGC1378550.37968086701477605No Hit
CCCCCCACTGCTAAATTTGACTGGCTT1351380.3721976932765791No Hit
TCCCTGAGACCCTAACTTGTGA1310500.3609385051125197No Hit
CGCGACCTCAGATCAGACGT1297840.3574516821634739No Hit
TGAGATGAAGCACTGTAGCTCT1253230.3451651757055804No Hit
CTTCTCACTACTGCACTTGACTAGTCTT1223890.33708433958196243No Hit
TATTGCACTTGTCCCGGCCTGTA1176330.3239853427844413No Hit
TATTGCACTTGTCCCGGCCTGTT1144310.3151663798438058No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1107670.3050749744051772No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGC1084570.2987127619152122No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTC1036630.2855091053451288No Hit
TGAGATGAAGCACTGTAGCTC1035010.28506292420946894No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC983800.27095864275444254No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC976980.2690802752574052No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT947020.2608286784522384No Hit
TGTGCAAATCTATGCAAAACTGA939720.2588181091372278No Hit
TATTGCACTTGTCCCGGCCTGTAT848770.23376861883582858No Hit
AACATTCAACGCTGTCGGTGAGT831730.22907545429777645No Hit
CAAAGTGCTTACAGTGCAGGTA831200.2289294814570976No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCG803880.22140499465078395No Hit
CGGGTGCTGTAGGCTT797930.21976624294882321No Hit
CGCGACCTCAGATCAGACG795880.2192016310178956No Hit
CCGGGTGCTGTAGGCTT780140.21486651307018784No Hit
TGAGATGAAGCACTGTAGCT759920.20929751148806258No Hit
CAAAGTGCTTACAGTGCAGGT737350.20308127183877636No Hit
TGAGGTAGTAGGTTGTGTGGTT720030.1983109895735731No Hit
ACCGGGTGCTGTAGGCTT718820.19797773082409878No Hit
TACAGTATAGATGATGTACT715610.1970936311664023No Hit
TAAAGTGCTTATAGTGCAGGTA711320.19591207741826594No Hit
CGGGTGCTGTAGGCTTT705290.19425129207997638No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC691160.19035960106338737No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC670040.18454272107256217No Hit
AAGCTGCCAGTTGAAGAACTGT653100.17987709857992112No Hit
TGAGGTAGTAGGTTGTATAGT652540.17972286312561894No Hit
GGGTGCTGTAGGCTTT633790.1745587296110369No Hit
AAACCGTTACCATTACTGAGTT605190.16668170462346113No Hit
CAAAGTGCTTACAGTGCAGGTAGT598980.16497134360343155No Hit
CAGTGCAATGTTAAAAGGGCAT596580.16431033451356505No Hit
TTCAAGTAATCCAGGATAGGCT589250.16229150258493114No Hit
TAGCTTATCAGACTGATGTTG578950.1594546719075874No Hit
CGCGACCTCAGATCAGA558850.15391872077995547No Hit
CGCGACCTCAGATCAGACGTGGCGAC553720.15250581385036582No Hit
TGAGGTAGTAGATTGTATAGT547940.150913883625604No Hit
TCGTACCGTGAGTAATAATGCG539160.14849569203850907No Hit
CCGGGTGCTGTAGGCTTT536080.14764739703984706No Hit
TCAAAGTGCTTACAGTGCAGGTAG533040.1468101188593495No Hit
CGCGACCTCAGATCAGAC532440.1466448665868829No Hit
ACTGCAGTGAAGGCACTTGTAG531850.14648236851895735No Hit
AGTTCTACAGTCCGACGATC528940.14568089499749423No Hit
CAGAGTTCTACAGTCCGACGATC511980.14100976409577096Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC508090.139938378529279No Hit
TCAGAGTTCTACAGTCCGACGATC504820.1390377536443359Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT482120.13278570933601527No Hit
CGCGACCTCAGATCAGACGTGGC480460.13232851138219093No Hit
TGAGGTAGTAGGTTGTATGGTT471480.1298552357042738No Hit
CAAAGTGCTGTTCGTGCAGGTAG465800.12829084752492306No Hit
CCTGTCTGAGCGTCGCT463230.1275830169578577No Hit
TGTAAACATCCTCGACTGGAAGCT459840.12664934161842126No Hit
CGTACGCGGGTTTAACGA458760.12635188752798135No Hit
TAAAGTGCTTATAGTGCAGGTAGT455130.12535211127955825No Hit
CCCCCCACTGCTAAATTTGACTGGCTTT448690.12357840355508315No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG442420.12185151730780694No Hit
ATCGCTTCTCGGCCTT438440.12075534390044498No Hit
TATTGCACTTGTCCCGGCCTGA436130.12011912265144847No Hit
ACCGGGTGCTGTAGGCTTT433770.11946913037974641No Hit
GGGTGCTGTAGGCTT426890.11757423765546245No Hit
TACAGTACTGTGATAACTGAAG418350.11522214697735417No Hit
AGGGAGAGAACGCGGTCTGAGTGGT410360.11302153754900693No Hit
CATTGCACTTGTCTCGGTCTGA398180.10966691641793445No Hit
CTGTCTGAGCGTCGCT397230.10940526698652896No Hit
GCCGCCGGTGAAATACCACTACTCTGA388230.10692648289952959No Hit
CGCGACCTCAGATCAGACGTG387930.10684385676329629No Hit
TGAGGTAGTAGTTTGTGCTGTT383910.1057366665377699No Hit
CTACAGTCCGACGATC381790.10515277517505449Illumina DpnII expression Sequencing Primer (100% over 16bp)
AAACCGTTACCATTACTGAGTTT379090.10440913994895468No Hit
CAAAGTGCTTACAGTGCAGGTAA377370.10393541676788369No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG374880.1032496198371472No Hit
GGCTGGTCCGAGTGCAGTGGTGTTT365030.10053672836415344No Hit
CGTACGCGGGTTAAACGA364930.10050918631874232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)