FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004043162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7545296
Sequences flagged as poor quality0
Sequence length13-34
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAGACCCTAACTTGTGA4997576.623424713887964No Hit
TCTTTGGTTATCTAGCTGTATGA3134414.154124636064642No Hit
TATTGCACTTGTCCCGGCCTGT1979902.6240189914351935No Hit
TAGCTTATCAGACTGATGTTGA1958192.5952460977011373No Hit
TAAGGCACGCGGTGAATGCCA1489501.9740776239924849No Hit
TGAGGTAGTAGGTTGTATAGTT1473081.9523157209471966No Hit
TCTTTGGTTATCTAGCTGTAT1076361.4265311791611621No Hit
TAGCTTATCAGACTGATGTTGAC1076241.4263721396748383No Hit
TGAGGTAGTAGGTTGTATGGTT804571.0663199959285892No Hit
AACCCGTAGATCCGATCTTGT791081.0484413070077039No Hit
TCTTTGGTTATCTAGCTGTATG765541.0145924030018172No Hit
CAGTGCAATGTTAAAAGGGCAT709140.939843844429695No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT632760.8386152113846825No Hit
CATTGCACTTGTCTCGGTCTGA622580.8251233616282251No Hit
TGAGGTAGTAGATTGTATAGTT614990.8150641141182533No Hit
TTCAAGTAATCCAGGATAGGCT595720.7895250232727781No Hit
CAAAGTGCTGTTCGTGCAGGTAG589440.7812019568218398No Hit
TGTGCAAATCCATGCAAAACTGA587050.7780344203858934No Hit
TGTAAACATCCTCGACTGGAAGCT568360.7532640203909826No Hit
AACATTCAACGCTGTCGGTGAGT563500.7468229211948743No Hit
TCTTTGGTTATCTAGCTGTATGT443340.5875713822227783No Hit
ACAGTAGTCTGCACATTGGTT404620.5362546413023426No Hit
AACCCGTAGATCCGATCTTGTG400850.5312581507736741No Hit
TCCCTGAGACCCTAACTTGTGAA384800.5099866194778839No Hit
TGAGGTAGTAGGTTGTATAGT370600.4911669469295837No Hit
TAAGGCACGCGGTGAATGCCAA367170.4866210682788323No Hit
TAAGGCACGCGGTGAATGCC357870.47429550808874826No Hit
ACAGTAGTCTGCACATTGGTTA351580.4659591883472829No Hit
TAGCTTATCAGACTGATGTTG344890.4570927369847386No Hit
TACCCTGTAGATCCGAATTTGT332450.44060564356918536No Hit
AGCTACATTGTCTGCTGGGTTTC327220.433674172623579No Hit
TTAAGGCACGCGGTGAATGCCA307860.4080158021633611No Hit
AACCCGTAGATCCGATCTTGTGT303930.4028072589862611No Hit
TGTAAACATCCTCGACTGGAAGC302090.4003686535292983No Hit
TCAGTGCACTACAGAACTTTGT301770.39994454823243514No Hit
TAAGGCACGCGGTGAATGC296650.39315886348262546No Hit
AACCCGTAGATCCGAACTTGTG292140.3871816294549611No Hit
TATGGCTTTTTATTCCTATGTGA291850.3867972840296789No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC289330.3834574548168819No Hit
TAAAGTGCTGACAGTGCAGATA287290.3807537835493796No Hit
TGAGGTAGTAGTTTGTGCTGTT286490.37969352030722187No Hit
TCCCTGAGACCCTAACTTGTG282780.37477654952171524No Hit
AATTGCACGGTATCCATCTGTA281860.3735572467932338No Hit
TGAGATGAAGCACTGTAGCTCT281580.3731861546584786No Hit
TGAGGTAGTAGTTTGTACAGTT275480.36510164743702567No Hit
TTCCCTTTGTCATCCTATGCCT267700.35479058740704145No Hit
CAAAGTGCTTACAGTGCAGGTAG262500.34789887633301597No Hit
TGTAAACATCCTTGACTGGAAGCT255210.3382372275388533No Hit
TAAAGTGCTTATAGTGCAGGTAG243590.3228369039465118No Hit
TTCAAGTAATCCAGGATAGGC241000.3194043017000261No Hit
AGCAGCATTGTACAGGGCTATGA236660.3136523736113202No Hit
CAAAGTGCTCATAGTGCAGGTAG236330.3132150150239301No Hit
CGTACGCGGGTTTAAACGA231120.3063100506593777No Hit
TAAAGTGCTGACAGTGCAGAT231100.30628354407832376No Hit
TCCCTGAGACCCTAACTTGT229780.30453410972876344No Hit
TTCACAGTGGCTAAGTTCTGC229730.3044678432761286No Hit
TGTGCAAATCTATGCAAAACTGA223910.2967544281894309No Hit
AACCCGTAGATCCGAACTTGT221160.2931097732945136No Hit
CCCAGTGTTCAGACTACCTGTTC216470.28689398003736366No Hit
TATACAAGGGCAAGCTCTCTGT209190.27724558453372805No Hit
TGAGGTAGTAGGTTGTGTGGTT207820.2754298837315329No Hit
TGTAAACATCCTACACTCTCAGCT187950.24909559545443943No Hit
TGAGATGAAGCACTGTAGCTC182640.2420580981846173No Hit
TATTGCACTCGTCCCGGCCTCC180970.23984479866661296No Hit
AGCTACATTGTCTGCTGGGTTT180920.2397785322139781No Hit
TCGGATCCGTCTGAGCTTGGCT179780.2382676570939033No Hit
TTGTGCTTGATCTAACCATGT175510.23260850203888622No Hit
CAGTGCAATGATGAAAGGGCAT174850.23173378486410606No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC174370.2310976269188114No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC173180.22952048534610175No Hit
TACAGTACTGTGATAACTGAAG169150.22417940926373203No Hit
TCCCTGAGACCCTTTAACCTGT165870.21983232997088517No Hit
TAAGGCACGCGGTGAATGCCAT165380.21918291873506357No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG162630.21553826384014624No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT162130.21487559931379763No Hit
AGAATTGTGGCTGGACATCTGT160180.2122912076610381No Hit
TGAGATGAAGCACTGTAGCT158050.20946825677879305No Hit
AACATTCAACGCTGTCGGTGAGTTT156840.20786460862502942No Hit
TTAAGGCACGCGGTGAATGCC151640.20097289755100395No Hit
GTACAGTACTGTGATAACTGA151260.200469272510979No Hit
TGTGCAAATCCATGCAAAACTG149660.1983487460266635No Hit
CACCCGTAGAACCGACCTTGCG145180.19241127187058005No Hit
TCGTACCGTGAGTAATAATGCG141210.18714971553137214No Hit
TAAGGCACGCGGTGAATGCCAAT139810.18529425485759604No Hit
TCACAGTGAACCGGTCTCTTT138880.18406169883858764No Hit
AACCCGTAGATCCGATCTTGTGA138590.18367735341330546No Hit
TGTAAACATCCTCGACTGGAAG138180.1831339685016996No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC137670.18245805068482404No Hit
ACAGTAGTCTGCACATTGGTTAA132590.17572537909712224No Hit
TACCCTGTAGAACCGAATTTGT129060.1710469675411011No Hit
AATTGCACGGTATCCATCTGT118020.15641533479932396No Hit
TGAGGTAGTAGATTGTATAGT116300.1541357688286848No Hit
TATTGCACTTGTCCCGGCCTGTA112380.14894047894211174No Hit
AACATTCAACCTGTCGGTGAGTTT108040.14318855085340587No Hit
AACATTCAACGCTGTCGGTGAGTT107690.14272468568496186No Hit
TGTAAACATCCCCGACTGGAAGCT106840.14159815599016926No Hit
AGCTCGGTCTGAGGCCCCTCAGT106780.14151863624700742No Hit
TCAGTGCATCACAGAACTTTGT106100.14061741249117332No Hit
TATGGCTTTTCATTCCTATGTGA105220.13945112292479978No Hit
TAGCAGCACGTAAATATTGGCG104740.1388149649795051No Hit
TGTAAACATCCTTGACTGGAAGC104340.13828483335842623No Hit
TTATCAGAATCTCCAGGGGTAC103560.1372510766973224No Hit
TATTGCACTTGTCCCGGCCTGTT102990.136495639137285No Hit
TGTAAACATCCTACACTCTCAGC102150.13538236273301935No Hit
TGGAAGACTAGTGATTTTGTTGTT100790.13357991522135115No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC98470.13050515181909364No Hit
CAACGGAATCCCAAAAGCAGCT96590.1280135332000229No Hit
TACAGTAGTCTGCACATTGGTT96050.12729785551156642No Hit
AACCCGTAGATCCGAACTTGTGA95960.12717857589682366No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGC93360.12373272035981094No Hit
TGAGGTAGTAGGTTGTATAGTTT93180.12349416113032544No Hit
TCCCTGAGACCCTAACTTGTGT90010.1192928680332753No Hit
CCCAGTGTTCAGACTACCTGTT87720.11625786450259872No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCT87530.11600605198258623No Hit
TAGCTTATCAGACTGATGTTGAA86310.11438915053829565No Hit
ATCATGGAGGAAAATCCACGT86080.11408432485617528No Hit
TCCCTGAGACCCTAACTTGTGAAA85720.11360720639720431No Hit
TTAAGGCACGCGGTGAATGC84910.11253368986451956No Hit
TGAGGTAGTAGGTTGTATGGT82350.10914084748961472No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC81340.10780226514639055No Hit
CTTCTCACTACTGCACTTGACTAGTCTT81270.10770949211270173No Hit
TCCCTGAGACCCTTTAACCTGTG80800.10708658745793406No Hit
TACAGTACTGTGATAACTGAAT79920.10592029789156052No Hit
TACTGCATCAGGAACTGATTGGA79470.10532389981784678No Hit
ATCACACAAAGGCAACTTTTGT78670.104263636575689No Hit
AAGCTGCCAGTTGAAGAACTGT78160.10358771875881344No Hit
AAATCTCTGCAGGCAAATGTGA76440.10130815278817426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)