FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004043496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14541572
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT10290047.07629133906568TruSeq Adapter, Index 27 (97% over 44bp)
GAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTC1224270.8419103519206864TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG933070.6416568992678371TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTAACTCGTAT726730.49976027351100694TruSeq Adapter, Index 27 (97% over 41bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT546910.3761010157636327Illumina Single End PCR Primer 1 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGC546760.37599786322964257TruSeq Adapter, Index 27 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTACCTCGTAT463520.3187550837007168TruSeq Adapter, Index 27 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGT360320.24778614031550372TruSeq Adapter, Index 27 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCG258180.1775461415038209TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTAGCTCGTAT207660.14280436805594335TruSeq Adapter, Index 27 (97% over 41bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC158180.10877778551039735Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)