FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004043497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004043497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14783754
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT10684947.227487686821629TruSeq Adapter, Index 27 (97% over 44bp)
GAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTC1284910.8691364859020245TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG976610.6605967604709873TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTAACTCGTAT718040.48569531121797616TruSeq Adapter, Index 27 (97% over 41bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT589460.3987214614095987Illumina Single End PCR Primer 1 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGC571500.3865729908655136TruSeq Adapter, Index 27 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTACCTCGTAT473340.3201757821457256TruSeq Adapter, Index 27 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGT372540.251992829426139TruSeq Adapter, Index 27 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCG266840.1804954276160169TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTAGCTCGTAT199190.13473573762117524TruSeq Adapter, Index 27 (97% over 41bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC167460.11327298871450377Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)