FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004044237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004044237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239178
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT463562.0702239839798353TruSeq Adapter, Index 27 (97% over 39bp)
GTCAGAGTGTAAGGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGG48180.21516824477553817No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36600.16345283849698417No Hit
GGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGGGAAGAGAGAAGT27620.12334883604608476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)