FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004044403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004044403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711121
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT143190.5281579095879527TruSeq Adapter, Index 6 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46530.1716264231659155No Hit
GTCAGAGTGTAAGGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGG32000.1180323563573887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)