FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004044449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004044449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105801
Sequences flagged as poor quality0
Sequence length35-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN256891.2199158420002651No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT208600.9905969272500108TruSeq Adapter, Index 6 (97% over 36bp)
GCCAGAAGGAGAGAGAAAGGAATGAGGGAAGAGAGAAGTGGGGAAGAAGG66520.3158892981815471No Hit
GGGCTACCACCCACCCTCTCAGTCTTGTGAAGAGTCTGGGACCTCCTTCT55660.26431747349345924No Hit
GTCAGAGTGTAAGGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGG30160.14322340999933042No Hit
GGCTACCACCCACCCTCTCAGTCTTGTGAAGAGTCTGGGACCTCCTTCTT22650.10756002110360857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)