FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004044497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004044497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2232438
Sequences flagged as poor quality0
Sequence length35-101
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN196550.8804275863428234No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT98860.4428342466845664TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGCGTAT77010.34495918811631054TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT67680.3031663141372795TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT59720.26751022872751673TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTAT59610.26701749387888934TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTTT45000.20157334716574435TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36800.16484220390443094No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGCGGAT35370.15843665087227507TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGTAT24450.10952151862672109TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)