FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004044541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004044541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784371
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT127870.45924196164950726TruSeq Adapter, Index 6 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58200.20902386930477299No Hit
GTCAGAGTGTAAGGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGG30530.10964774449956562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)