FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004044617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004044617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294213
Sequences flagged as poor quality0
Sequence length35-101
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATCTCGTAT304771.3284294004087678TruSeq Adapter, Index 7 (97% over 35bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN247601.079237193756639No Hit
GGGAGGTTCCTGTGTGGATGGGGAAAATGGCTTCCGCTGCCTCTGCCCGC31170.13586358372130225No Hit
GGGCTACCACCCACCCTCTCAGTCTTGTGAAGAGTCTGGGACCTCCTTCT29700.12945615773252092No Hit
GCCAGAAGGAGAGAGAAAGGAATGAGGGAAGAGAGAAGTGGGGAAGAAGG24580.10713913660152742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)