FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004044743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004044743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082943
Sequences flagged as poor quality0
Sequence length35-101
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATCTCGTAT29499727.240307199917265TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12713611.739860731358899No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66210.6113895191159646No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATGTCGTAT63440.5858110722355655TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCAGCTCGTAT41210.380537110448103TruSeq Adapter, Index 7 (97% over 35bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32990.3046328384781101No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATATCGTAT14730.13601823918710404TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACGAATAATCATCTCGTATG13390.12364455008250666Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATCGCGTAT10980.1013903778869248TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)