FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004044749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004044749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113461
Sequences flagged as poor quality0
Sequence length35-101
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATCTCGTAT29994626.938168467508067TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13057811.727218106426719No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78560.705547836879783No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATGTCGTAT65160.5852023555382722TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCAGCTCGTAT40280.3617549245101535TruSeq Adapter, Index 7 (97% over 35bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37040.3326564648425046No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATATCGTAT14910.13390680050760648TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACGAATAATCATCTCGTATG13010.11684288897410866Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAATCCAGTCACGAATAATCATCTCGTATG11440.1027427094437973Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)