FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004044999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004044999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2216306
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT459782.0745330292838626TruSeq Adapter, Index 27 (97% over 39bp)
GTCAGAGTGTAAGGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGG47320.21350842347581966No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31900.14393319334063076No Hit
GGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGGGAAGAGAGAAGT26210.11825984318049945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)