FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004045001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004045001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231275
Sequences flagged as poor quality0
Sequence length35-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN318991.4296310405485653No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT187200.8389821962779128TruSeq Adapter, Index 6 (97% over 36bp)
GCCAGAAGGAGAGAGAAAGGAATGAGGGAAGAGAGAAGTGGGGAAGAAGG70090.314125331929054No Hit
GGGCTACCACCCACCCTCTCAGTCTTGTGAAGAGTCTGGGACCTCCTTCT58770.2633920068122486No Hit
GTCAGAGTGTAAGGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGG31580.14153342819688294No Hit
GGCTACCACCCACCCTCTCAGTCTTGTGAAGAGTCTGGGACCTCCTTCTT24390.10930969961120884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)