FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004045021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004045021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489797
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT128300.36764316090592086TruSeq Adapter, Index 19 (97% over 38bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52510.15046720482595405No Hit
CCCAAATATAAAGGTAGGGAAAGGGACAAGAGGGAACATACCCCTTAGTG37430.10725552231261588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)