FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004045093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004045093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080079
Sequences flagged as poor quality0
Sequence length35-101
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATCTCGTAT29790327.58159356861859TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11486610.634962812905352No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68710.6361571699847882No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATGTCGTAT66930.6196768940049756TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCAGCTCGTAT43340.4012669443624032TruSeq Adapter, Index 7 (97% over 35bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15880.147026282336755No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATATCGTAT14780.13684184212451128TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACGAATAATCATCTCGTATG13590.12582412953126576Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)