FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004045101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004045101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275199
Sequences flagged as poor quality0
Sequence length35-101
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATCTCGTAT299761.3175111275980695TruSeq Adapter, Index 7 (97% over 35bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN243991.0723897118449859No Hit
GGGAGGTTCCTGTGTGGATGGGGAAAATGGCTTCCGCTGCCTCTGCCCGC30520.13414211240423365No Hit
GGGCTACCACCCACCCTCTCAGTCTTGTGAAGAGTCTGGGACCTCCTTCT29230.12847227868858943No Hit
GCCAGAAGGAGAGAGAAAGGAATGAGGGAAGAGAGAAGTGGGGAAGAAGG23920.1051336608358214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)