FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004045107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004045107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179039
Sequences flagged as poor quality0
Sequence length35-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN270971.243529831269656No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT216130.9918592553873519TruSeq Adapter, Index 6 (97% over 36bp)
GCCAGAAGGAGAGAGAAAGGAATGAGGGAAGAGAGAAGTGGGGAAGAAGG67700.31068741771028424No Hit
GGGCTACCACCCACCCTCTCAGTCTTGTGAAGAGTCTGGGACCTCCTTCT57040.2617667696631405No Hit
GTCAGAGTGTAAGGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGG30580.14033709355362617No Hit
GGCTACCACCCACCCTCTCAGTCTTGTGAAGAGTCTGGGACCTCCTTCTT23650.10853408314399145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)