FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004045115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004045115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12480518
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGGT398330.31916143224183485TruSeq Adapter, Index 8 (97% over 36bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213240.17085829290098375No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGTT197730.15843092410106696TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGGG145150.11630126249567525TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128990.1033530819794499No Hit

[OK]Adapter Content

Adapter graph