FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004045327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004045327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671584
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATCTCGTAT271651.625105289354289TruSeq Adapter, Index 7 (97% over 35bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83710.5007824913375577No Hit
GTCAGAGTGTAAGGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGG22260.1331671037770163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)