FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004045457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004045457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108417
Sequences flagged as poor quality0
Sequence length35-101
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATCTCGTAT30223427.267174718540044TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12912111.649135659232943No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79260.7150738395387295No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71070.6411846804947957No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATGTCGTAT65180.5880458347354831TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCAGCTCGTAT41250.372152357822011TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATAATCATATCGTAT15610.14083147407519012TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACGAATAATCATCTCGTATG13830.12477253596796152Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)